TROY/TNFRSF19 Antibody [Alexa Fluor® 594]

• Applications: WB, IHC, ELISA(Cap), ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

TROY/TNFRSF19 Antibody [Alexa Fluor® 594] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TROY/TNFRSF19
  Glu30-Leu170
  Accession # Q9JLL3
• Specificity: Detects mouse TROY/TNFRSF19 in ELISAs and Western blots. In sandwich immunoassays, less than 2% cross-reactivity with recombinant human TAJ is observed and less than 0.2% cross-reactivity with recombinant mouse (rm) EDAR, rmCD27, rmFas, rmRANK,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

TROY, also named Toxicity and JNK inducer (TAJ) and TNFRSF19, is a novel member of the tumor necrosis factor receptor superfamily. The protein putatively encoded by the TROY mRNA is a 416 amino acid (aa) residue type I membrane protein with a 29 aa signal peptide, a 141 aa extracellular domain, a 23 aa transmembrane domain and a 223 aa cytoplasmic domain. Splice variants of mouse TROY (lacking the transmembrane and cytoplasmic domains or with only a 21 aa cytoplasmic tail), have also been identified. Like other members of the TNFRSF, TROY contains characteristic cysteine-rich motifs in the extracellular domain. The cytoplasmic domain of TROY does not have the death domain present in some TNFRSF members, but does contain a major TRAF2 (tumor necrosis factor
receptor‑associated factor 2)-binding consensus sequence. Mouse TROY shares 92% aa sequence identity with human TROY in their extracellular domains. The two proteins also have 57% homology in their cytoplasmic tails. Among TNFRSF members, TROY is most related to Edar, sharing 33% identity in the extracellular domain. Over‑expression of TROY has been reported to activate nuclear factor NF kappa B and c-Jun N-terminal kinase (JNK) pathways. TROY mRNA is exclusively expressed in the epithelia of various tissues in mouse day 13.5 embryos. In neonatal mice, expression of TROY is predominantly in hair follicles and in neuron-like cells in the cerebrum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.