tropomyosin-3 Overexpression Lysate

Western Blot: tropomyosin-3 Overexpression Lysate (Adult Normal) [NBL1-17220] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for tropomyosin-3.

• Reactivity: Hu
• Applications: WB

tropomyosin-3 Overexpression Lysate Summary

• Description:

  tropomyosin-3 Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC203276
  
  Accession#: NM_152263
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: TPM3

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 32.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for tropomyosin-3 Overexpression Lysate

• cytoskeletal tropomyosin TM30
• FLJ41118
• gamma-tropomyosin
• heat-stable cytoskeletal protein 30 kDa
• hscp30
• hTM5
• MGC14582
• MGC3261
• MGC72094
• NEM1
• OK/SW-cl.5
• TM3
• TM30
• TM30nm
• TM-5
• TPMsk3
• TRK
• tropomyosin 3
• tropomyosin alpha-3 chain
• tropomyosin gamma
• tropomyosin-3
• tropomyosin-5

Background

This gene encodes a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) differs in the 5' and 3' UTRs and has multiple coding region differences, compared to variant 2. The resulting isoform (1), also known as HMW or slow-muscle, is longer and differs in a central region and at the N- and C-termini, compared to isoform 2. This variant is transcribed from the distal promoter, and is predominantly expressed in slow-twitch skeletal muscle. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: TPM3