TrkA Antibody

Product Discontinued

TrkA Antibody Summary

• Immunogen: Extracellular domain of glycosylated human Trk A protein produced in CHO cells was used as the immunogen.
• Localization: Accession Number: NTRK1_MUS
• Specificity: Specificity was demonstrated by immunohistochemistry. When used for immunohistochemistry in rat dorsal root ganglia, staining is restricted to the known distribution of TrkA, that is in small, nociceptive neurons.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NTRK1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000 (1-site)
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:1000
• Application Notes: Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25662281)
• Control:

  TrkA Overexpression Lysate

Reactivity Notes

Reacts with human, rat and mouse. Other species have not yet been tested.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute with deionized water.

Alternate Names for TrkA Antibody

• DKFZp781I14186
• EC 2.7.10
• EC 2.7.10.1
• MTChigh affinity nerve growth factor receptor
• Neurotrophic tyrosine kinase receptor type 1
• neurotrophic tyrosine kinase, receptor, type 1
• NTRK1
• NTRK-1
• p140-TrkA
• TRK1-transforming tyrosine kinase protein
• TrkA
• Trk-A
• TRKAOncogene TRK
• TRKTRK1
• tyrosine kinase receptor A

Background

TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.



This product is distributed on behalf of Biosensis Pty. Ltd. of Australia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TrkA Antibody (R-152-100)(4)

Publications using R-152-100

• Ceriani M, Amigoni L, D'Aloia A et al. The deubiquitinating enzyme UBPy/USP8 interacts with TrkA and inhibits neuronal differentiation in PC12 cells Exp. Cell Res. 2015-02-04 [PMID: 25662281] (ICC/IF, Rat)
  
  Details:
  TrkA antibody R-152-100 used at 1:400 dilution in ICC-IF application on PC12 cells - fixation with 3.7% paraformaldehyde-PBS for 10 minutes, permeabilization with 0.1% Triton X-100 -PBS for 4 minutes, detection with Alexa Fluor 568 goat anti-rabbit IgG secondary antibody (Fig 5B).
• Matrone C, Marolda R, Ciafre S et al. Tyrosine kinase nerve growth factor receptor switches from prosurvival to proapoptotic activity via Abeta-mediated phosphorylation. PNAS;106(27):11358-11363. 2009-01-01 [PMID: 19549834]
• Zhang FX et al. Brain Res 16; 1062 (1-2) pp. 92-100. 2005-01-01 [PMID: 16256078]
• Gearhart DA et al. J Neurosci Methods. 150(2) pp. 159-173. 2006-01-01 [PMID: 16085318]

FAQs for TrkA Antibody (R-152-100). (Showing 1 - 1 of 1 FAQs).

1. I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.
   • NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

Bioinformatics

• Gene Symbol: NTRK1
• Entrez:
  • 4914(Human)
  • 18211(Mouse)
  • 59109(Rat)
• Uniprot:
  • P04629()