TRIP6 Antibody

Western Blot: TRIP6 Antibody [NB100-79791] - Detection of Human TRIP6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TRIP6 antibody NB100-79791 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Western Blot: TRIP6 Antibody [NB100-79791] - Nuclear extract (NE) from HeLa cells. Antibody used at 0.3 ug/ml.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

TRIP6 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 100 and 150 of human Thyroid Hormone Receptor Interactor 6 using the numbering given in entry NP_003293.1 (GeneID 7205).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIP6
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:10000
• Control:

  TRIP6 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIP6 Antibody

• MGC10556
• MGC10558
• MGC29959
• MGC3837
• MGC4423
• OIP1OIP-1
• Opa-interacting protein 1
• thyroid hormone receptor interactor 6
• thyroid receptor-interacting protein 6
• TR-interacting protein 6
• ZRP-1TRIP-6
• zyxin related protein 1
• Zyxin-related protein 1

Background

This gene is a member of the zyxin family and encodes a protein with three LIM zinc-binding domains. This protein localizes to focal adhesion sites and along actin stress fibers. Recruitment of this protein to the plasma membrane occurs in a lysophosphatidic acid (LPA) dependent manner and it regulates LPA-induced cell migration. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIP6 Antibody (NB100-79791)(5)

Publications using NB100-79791

• Lai YJ, Lin VT, Zheng Y et al. The adaptor protein TRIP6 antagonizes Fas-induced apoptosis but promotes its effect on cell migration. Mol Cell Biol 2010-12-01 [PMID: 20876301]
• E S, Lai YJ, Tsukahara R et al. Lysophosphatidic acid 2 receptor-mediated supramolecular complex formation regulates its antiapoptotic effect. J Biol Chem 2009-05-01 [PMID: 19293149]
• Lai YJ, Lin WC, Lin FT et al. PTPL1/FAP-1 negatively regulates TRIP6 function in lysophosphatidic acid-induced cell migration. J Biol Chem 2007-08-01 [PMID: 17591779]
• Solaz-Fuster MC, Gimeno-Alcaniz JV, Casado M et al. TRIP6 transcriptional co-activator is a novel substrate of AMP-activated protein kinase. Cell Signal 2006-10-01 [PMID: 16624523]
• Li L, Bin LH, Li F et al. TRIP6 is a RIP2-associated common signaling component of multiple NF-kappaB activation pathways. J Cell Sci 2005-02-01 [PMID: 15657077]

Bioinformatics

• Gene Symbol: TRIP6
• Entrez:
  • 7205(Human)
• Uniprot:
  • NP_003293.1(Human)