Western Blot: Ajuba Antibody [NBP1-89570] - Analysis in human cell line HepG2.
Immunocytochemistry/ Immunofluorescence: Ajuba Antibody [NBP1-89570] - Staining of human cell line U-251 MG shows localization to nucleoplasm & the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ajuba Antibody [NBP1-89570] - Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Ajuba Antibody [NBP1-89570] - Staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
Immunohistochemistry-Paraffin: Ajuba Antibody [NBP1-89570] - Staining of human Fallopian tube shows strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: Ajuba Antibody [NBP1-89570] - Staining of human kidney shows moderate nuclear positivity in cells in tubules.
Immunohistochemistry-Paraffin: Ajuba Antibody [NBP1-89570] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
This antibody was developed against Recombinant Protein corresponding to amino acids: LDRLYAQRPAGFGCQESRHSYPPALGSPGALAGAGVGAAGPLERRGAQPGRHSVTGYGDCAVGARYQDELTALLRLTVGTGGREAGARGEPSGIEPSGLEEPPGPFVPEAARARMREPEAREDYFGTCIKCNKGIYGQSN
Mouse (91%). Backed by our 100% Guarantee.
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
57 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%). Human reactivity reported in scientific literature (PMID: 25961170).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for Ajuba Antibody
jub, ajuba homolog (Xenopus laevis)
Ajuba is a LIM domain protein suggested to bind and regulate the activity of Aurora A. Aurora A, which is involved in cell cycle regulation, is upregulated during mitosis, localizing to the centrosomes and microtubule regions proximal to the centrosomes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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