Recombinant Human Triosephosphate isomerase His Protein

SDS-Page: Recombinant Human Triosephosphate isomerase Protein [NBP2-53086] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE, Enzyme Activity
• Concentration: 0.5 mg/ml

Recombinant Human Triosephosphate isomerase His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-249 of Human Triosephosphate isomerase
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ

• Details of Functionality: Specific activity is > 3000 units/mg, in which one unit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetone phosphate per minute at pH 7.5 at 25C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TPI1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 28.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human Triosephosphate isomerase His Protein

• EC 5.3.1.1
• MGC88108
• TIM
• TPI
• triosephosphate isomerase 1
• triosephosphate isomerase
• Triose-phosphate isomerase

Background

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TPI1