Triosephosphate isomerase Antibody

Western Blot: Triosephosphate isomerase Antibody [NB300-994] - Staining (0.001ug/ml) of HepG2 (A), HEK293 (B), HeLa (C) and Jurkat (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NB300-994] - Staining of paraffin embedded Human Liver. Antibody at 2 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Flow Cytometry: Triosephosphate isomerase Antibody [NB300-994] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western Blot: Triosephosphate isomerase Antibody [NB300-994] - Staining (0.001ug/ml) of RAW264.7 (A), KNRK (B) and MDCK (C) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody [NB300-994] - Staining of paraffin embedded Human Pancreas. Antibody at 5 ug/mL. Steamed antigen retrieval with Tris/EDTA buffer pH 9, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca, Bv
• Applications: WB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Triosephosphate isomerase Antibody Summary

• Immunogen: Peptide with sequence C-LKPEFVDIINAKQ corresponding to C-Terminus according to NP_000356.1; NP_001152759.1; NP_001244955.1.
• Specificity: This antibody is expected to recognise reported isoforms 1, 2 and 3 (NP_000356.1; NP_001152759.1, NP_001244955.1)
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunohistochemistry 2 - 4 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.001 - 0.01 ug/mL
• Application Notes: Approx 26 kDa band observed in HEK293, HeLa, HepG2, Jurkat, Raw264.7. KNRK and MDCK lysates (calculated MW of 26.7kDa according to NP_000356.1).
• Theoretical MW: 26.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Triosephosphate isomerase Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Triosephosphate isomerase Antibody

• EC 5.3.1.1
• MGC88108
• TIM
• TPI
• triosephosphate isomerase 1
• triosephosphate isomerase
• Triose-phosphate isomerase

Background

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Triosephosphate isomerase Antibody (NB300-994)(2)

Publications using NB300-994

• Govekar RB, D'Cruz AK, Alok Pathak K et al. Proteomic profiling of cancer of the gingivo-buccal complex: Identification of new differentially expressed markers. Proteomics Clin Appl. 2009-12-01 [PMID: 21136964] (WB, Human)
  
  Details:
  Triosephosphate Isomerase (IMG-3793). WB: Human gingivo squamous complex tissue and normal tissue, Fig 1.
• Jiang PZ, Gan M, Huang H et al. Proteomics-based Identification of Proteins with Altered Expression Induced by 12-O-Tetradecanoylphorbol 13-acetate in Nasopharyngeal Carcinoma CNE2 Cells. Acta Biochim Biophys Sin (Shanghai) 2005-02-01 [PMID: 15685366]

Bioinformatics

• Gene Symbol: TPI1
• Entrez:
  • 7167(Human)
  • 21991(Mouse)
  • 24849(Rat)
• Uniprot:
  • P60174(Human)