Triosephosphate isomerase Antibody (1D10-2E2) - Azide and BSA Free

Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - TPI1 monoclonal antibody (M01), clone 1D10-2E2 Analysis of TPI1 expression in HepG2.

Western Blot: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - Antibody Reactive Against Recombinant TPI1 (AAH07086, 1 a.a. ~ 250 a.a) ) full length protein with GST tag (53.5 KDa) .

Sandwich ELISA: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - Detection limit for recombinant GST tagged FLJ23834 is approximately 0.1ng/ml as a capture antibody.

Sandwich ELISA: Triosephosphate isomerase Antibody (1D10-2E2) [H00007167-M01] - Detection limit for recombinant GST tagged TPI1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: 1D10-2E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Triosephosphate isomerase Antibody (1D10-2E2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TPI1 (AAH07086.1, 1 a.a. ~ 249 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAPSRKFFVGGNWKMNGRKQSLGELIGTLNAAKVPADTEVVCAPPTAYIDFARQKLDPKIAVAAQNCYKVTNGAFTGEISPGMIKDCGATWVVLGHSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKVIADNVKDWSKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKSNVSDAVAQSTRIIYGGSVTGATCKELASQPDVDGFLVGGASLKPEFVDTINAKQ
• Localization: Cytoplasmic
• Specificity: TPI1 - triosephosphate isomerase 1
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPI1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Triosephosphate isomerase Antibody (1D10-2E2) - Azide and BSA Free

• EC 5.3.1.1
• MGC88108
• TIM
• TPI
• triosephosphate isomerase 1
• triosephosphate isomerase
• Triose-phosphate isomerase

Background

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Triosephosphate isomerase Antibody (H00007167-M01)(4)

Publications using H00007167-M01

• Alma O, Oscar O, Adriana A et al. Serum Analysis of Women with Early-Stage Breast Cancer Using a Mini-Array of Tumor-Associated Antigens. Biosensors (Basel). 2020-10-21 [PMID: 33096879]
• Vasko R, Mueller GA, von Jaschke AK et al. Impact of cisplatin administration on protein expression levels in renal cell carcinoma: A proteomic analysis. Eur J Pharmacol. 2011-09-08 [PMID: 21924258]
• Fung KY, Lewanowitsch T, Henderson ST et al. Proteomic Analysis of Butyrate Effects and Loss of Butyrate Sensitivity in HT29 Colorectal Cancer Cells. J Proteome Res. 2009-02-05 [PMID: 19195990]
• Lee CL, Jiang PP, Sit WH et al. Proteome of human T lymphocytes with treatment of cyclosporine polysaccharopeptide: Analysis of significant proteins that manipulate T cells proliferation immunosuppression. Int Immunopharmacol;7(10):1311-24. 2007-10-01 [PMID: 17673146]

Bioinformatics

• Gene Symbol: TPI1
• Entrez:
  • 7167(Human)
• OMIM:
  • 190450(Human)
• Uniprot:
  • AAH07086(Human)