Triosephosphate isomerase Antibody (181) - Azide and BSA Free

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Triosephosphate isomerase antibody [181] diluted at 1:500.

Immunocytochemistry/Immunofluorescence: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Triosephosphate isomerase protein stained by Triosephosphate isomerase antibody [181] diluted at 1:200. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Mouse testis. Triosephosphate isomerase stained by Triosephosphate isomerase antibody [181] diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Analysis of A. 30 ug 293T whole cell extract B. 30 ug A431 whole cell extract C. 30 ug HeLa whole cell extract D. 30 ug HepG2 whole cell extract 12 % SDS-PAGE Triosephosphate isomerase antibody [181] dilution: 1:1000

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Analysis of A. 30 ug Neuro2A whole cell extract B. 30 ug C8D30 whole cell extract C. 30 ug NIH-3T3 whole cell extract D. 30 ug Raw264.7 whole cell extract E. 30 ug C2C12 whole cell extract 12 % SDS-PAGE Triosephosphate isomerase antibody [181] dilution: 1:1000

Western Blot: Triosephosphate isomerase Antibody (181) [NBP2-43761] - Analysis of A. 30 ug PC-12 whole cell extract B. 30 ug Rat2 whole cell extract 12 % SDS-PAGE Triosephosphate isomerase antibody [181] dilution: 1:1000

• Reactivity: Hu, Mu, Rt, Bv, Ca, Pm
• Applications: WB, ICC/IF, IHC
• Clone: 181
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Triosephosphate isomerase Antibody (181) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Triosephosphate isomerase Antibody (181) - Azide and BSA Free (NBP2-43761) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Triosephosphate isomerase Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.
• Predicted Species: Bovine (100%), Chimpanzee (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPI1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 31 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for Triosephosphate isomerase Antibody (181) - Azide and BSA Free

• EC 5.3.1.1
• MGC88108
• TIM
• TPI
• triosephosphate isomerase 1
• triosephosphate isomerase
• Triose-phosphate isomerase

Background

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Triosephosphate isomerase Antibody (NBP2-43761)(2)

Publications using NBP2-43761

• Tang Q, Tang Y, Yang Q et al. Embelin attenuates lipopolysaccharide-induced acute kidney injury through the inhibition of M1 macrophage activation and NF-?B signaling in mice Heliyon 2023-02-01 [PMID: 36938407] (IHC-P, Mouse)
• Tang Y, Wang C, Chen S et al. Dimethyl fumarate attenuates LPS induced septic acute kidney injury by suppression of Nf kappa B p65 phosphorylation and macrophage activation International immunopharmacology 2022-01-01 [PMID: 34915410] (ICC/IF, Mouse)

Bioinformatics

• Gene Symbol: TPI1