TRIM8 Antibody

Flow Cytometry: TRIM8 Antibody [NB100-1192] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunohistochemistry-Paraffin: TRIM8 Antibody [NB100-1192] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

Immunocytochemistry/Immunofluorescence: TRIM8 Antibody [NB100-1192] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: TRIM8 Antibody [NB100-1192] - (4ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Immunocytochemistry/Immunofluorescence: TRIM8 Antibody [NB100-1192] - Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Reactivity: Hu, Mu, Rt, Bv
• Applications: ELISA, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIM8 Antibody Summary

• Description: Novus Biologicals Goat TRIM8 Antibody (NB100-1192) is a polyclonal antibody validated for use in IHC, ELISA, Flow and ICC/IF. Anti-TRIM8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-YGQPSTKHYVTS, from the C Terminus of the protein sequence according to NP_112174.2; NP_001332879.1.
• Predicted Species: Mouse (100%), Rat (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 4 ug/ml
  • Peptide ELISA Detection limit 1:128000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM8 Antibody

• EC 6.3.2.-
• GERP
• GERPRING finger protein 27glioblastoma expressed ring finger protein
• Glioblastoma-expressed RING finger protein
• RNF27probable E3 ubiquitin-protein ligase TRIM8
• tripartite motif containing 8
• tripartite motif protein TRIM8
• tripartite motif-containing 8
• Tripartite motif-containing protein 8

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to nuclear bodies. Its structure is similar to some tumor suppressor proteins and its gene maps to a locus thought to contain tumor suppressor genes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM8 Antibody (NB100-1192)(1)

Publication using NB100-1192

• Vincent SR, Kwasnicka DA, Fretier P. A novel RING finger-B box-coiled-coil protein, GERP. Biochem Biophys Res Commun 2000-12-20 [PMID: 11118312]

Bioinformatics

• Gene Symbol: TRIM8
• Entrez:
  • 81603(Human)
  • 93679(Mouse)
• Uniprot:
  • NP_112174.(Human)