TRIM72 Antibody (HL1853) - Azide and BSA Free

Immunohistochemistry-Paraffin: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - TRIM72 antibody [HL1853] detects TRIM72 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. TRIM72 stained by TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - TRIM72 antibody [HL1853] detects TRIM72 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. TRIM72 stained by TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - Various whole cell extracts (10 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: TRIM72 Antibody (HL1853) - Azide and BSA Free [NBP3-25729] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM72 antibody [HL1853] (NBP3-25729) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Rb
• Applications: WB, IHC
• Clone: HL1853
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TRIM72 Antibody (HL1853) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit TRIM72 Antibody (HL1853) - Azide and BSA Free (NBP3-25729) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human TRIM72.
• Source: HEK293
• Predicted Species: Rabbit (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TRIM72
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for TRIM72 Antibody (HL1853) - Azide and BSA Free

• MG53Mg53
• Mitsugumin-53
• tripartite motif containing 72
• tripartite motif-containing 72
• tripartite motif-containing protein 72

Background

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of therepair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membranedamage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization atthe injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to theinjury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealingprocess. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membranebudding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (Bysimilarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM72