TRIM5 delta Antibody - BSA Free

Images

 
Western Blot: TRIM5 delta Antibody [NBP1-76603] - Analysis of TRIM5 delta in human kidney tissue lysate with TRIM5 delta antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: TRIM5 delta Antibody [NBP1-76603] - Staining of mouse brain tissue at 10 ug/mL.
Immunohistochemistry-Paraffin: TRIM5 delta Antibody [NBP1-76603] - Mouse brain tissue with TRIM5 delta antibody at 1 ug/ml.
Western Blot: TRIM5 delta Antibody [NBP1-76603] - Mouse brain cell lysate with TRIM5 delta antibody at 2 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

TRIM5 delta Antibody - BSA Free Summary

Immunogen
Antibody was raised against a synthetic peptide corresponding to 13 amino acids near the carboxy terminus of human TRIM5 delta. The immunogen is located within the last 50 amino acids of TRIM5 delta. Amino Acid Squence: SAMARSRFTATSTS
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM5
Purity
Peptide affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 10 ug/ml
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Western Blot 2 ug/ml
Application Notes
TRIM5 delta antibody can be used for detection of TRIM5 delta by Western blot at 1 to 2 mg/ml. A band at approximately 25 kDa can be detected.
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for TRIM5 delta Antibody - BSA Free

  • EC 6.3.2
  • EC 6.3.2.-
  • RING finger protein 88
  • RNF88tripartite motif protein TRIM
  • TRIM5alpha
  • tripartite motif containing 5
  • tripartite motif protein TRIM5
  • tripartite motif-containing 5
  • tripartite motif-containing protein 5

Background

TRIM5 is a member of a broad family of otherwise unrelated proteins defined by the presence of a tripartite motif containing a RING domain, a B-box type 1, and a B-box type 2, followed by a coiled-coil region. TRIM5 has five alternately spliced isoforms, the longest of which is the alpha variant which also contains a carboxy-terminal B30.2 (SPRY) domain. Expression of TRIM5alpha variants from humans, rhesus monkeys, and African green monkeys enabled resistance to infection by various retroviruses including HIV-1 , albeit at differing efficiencies. The TRIM5delta isoform has been shown to colocalize with the topoisomerase I-interacting proteins BTBD1 and BTBD2 in punctate or elongated cytoplasmic bodies in several mouse and human cells where it appears to serve as a scaffold for the assembly of endogenous BTBD proteins. TRIM5delta also exhibits autoubiquitination activity in a RING finger- and UbcH5B-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM5
Uniprot