TRIM4 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: TRIM4 Antibody [NBP1-80824] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Immunohistochemistry-Paraffin: TRIM4 Antibody [NBP1-80824] - Staining of human bone marrow shows distinct cytoplasmic positivity in hematopoietic cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRIM4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KSQRNLVAKMKKVMHLQDVEVKNATQWKDKIKSQRMRISTEFSKLHNFLVEEEDLFLQRLNKEEEETKKKLNENTLKLNQTIASLKKLILEVGEKSQAPTLELLQNPK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  TRIM4 Protein (NBP1-80824PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for TRIM4 Antibody - BSA Free

• RING finger protein 87
• RNF87tripartite motif protein 4
• tripartite motif containing 4
• tripartite motif-containing 4
• tripartite motif-containing protein 4

Background

TRIM4 is encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Alternative splicing of this gene generates two transcript variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM4 Antibody (NBP1-80824)(2)

Publications using NBP1-80824

• Stupin A Atg1 modulates mitochondrial dynamics to promote germline stem cell maintenance in Drosophila Biochemical and Biophysical Research Communications 2022-11-01 [PMID: 36621115] (IF/IHC)
  
  Details:
  Drosophila expressing GFP
• Ye S, Fang L, Xie S et al. Resveratrol alleviates postpartum depression-like behavior by activating autophagy via SIRT1 and inhibiting AKT/mTOR pathway Behavioural brain research 2022-11-07 [PMID: 36356720] (WB, IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: TRIM4
• Entrez:
  • 89122(Human)
• Uniprot:
  • Q9C037(Human)