ARPC1A Antibody - BSA Free

Western Blot: ARPC1A Antibody [NBP1-88851] - Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1, using anti-ARPC1A antibody. Remaining relative intensity is presented. Loading control: anti-PPIB.

Immunocytochemistry/Immunofluorescence: ARPC1A Antibody [NBP1-88851] - Immunofluorescent staining of human cell line MCF7 shows localization to cytosol & cell junctions. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ARPC1A Antibody [NBP1-88851] - Staining of human kidney shows strong membranous and cytoplasmic positivity in cells in distal tubules.

Immunohistochemistry-Paraffin: ARPC1A Antibody [NBP1-88851] - Staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: ARPC1A Antibody [NBP1-88851] - Staining of human fallopian tube shows moderate membranous and cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ARPC1A Antibody [NBP1-88851] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, KD
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARPC1A Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ARPC1A Antibody - BSA Free (NBP1-88851) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ARPC1A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HGVSFSASGSRLAWVSHDSTVSVADASKSVQVSTLKTEFLPLLSVSFVSENSVVAAGHDCCPMLFNYDDRGCLTFVSKLDIPKQSIQRNMSAMERFRNMDKRATTEDRNTALETLHQNSITQVSIYEVDKQDCRKFCTTGI
• Predicted Species: Mouse (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARPC1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Knockdown Validated
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  ARPC1A Protein (NBP1-88851PEP)

Reactivity Notes

Rat (84%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ARPC1A Antibody - BSA Free

• actin binding protein (Schizosaccharomyces pombe sop2-like)
• actin related protein 2/3 complex, subunit 1A (41 kD)
• actin related protein 2/3 complex, subunit 1A, 41kDa
• actin-related protein 2/3 complex subunit 1A
• SOP2Hs
• SOP2LArc40
• SOP2-like protein

Background

The ARPC1A gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they bo

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARPC1A Antibody (NBP1-88851)(1)

Publication using NBP1-88851

• Jasmine V. G. Abella, Chiara Galloni, Julien Pernier et al. Isoform diversity in the Arp2/3 complex determines actin filament dynamics. Nature Cell Biology 2016-01-18 [PMID: 26655834] (ICC/IF, WB, Human)

Bioinformatics

• Gene Symbol: ARPC1A
• Entrez:
  • 10552(Human)
• Uniprot:
  • Q92747(Human)