Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

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Western blot shows lysates of human prostate cancer tissue. PVDF Membrane was probed with 1 µg/mL of Human Cytosolic Sulfotransferase 2B1/SULT2B1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6174) ...read more
Cytosolic Sulfotransferase 2B1/SULT2B1 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Human Cytosolic Sulfotransferase 2B1/SULT2B1 Antigen Affinity-purified Polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody Summary

Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 2B1/SULT2B1 isoform 1
Asp2-Ser365
Accession # O00204
Specificity
Detects human SULT2B1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) SULT1B1, rhSULT1A1, rhSULT2A1, rhSULT4A1, and rhSULT1E1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SULT2B1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Immunoprecipitation 25 ug/mL
Publications
Read Publication using
AF6174 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody

  • Alcohol sulfotransferase
  • Cytosolic Sulfotransferase 2B1
  • EC 2.8.2
  • EC 2.8.2.2
  • HSST2
  • HSST2sulfotransferase family cytosolic 2B member 1
  • Hydroxysteroid sulfotransferase 2
  • ST2B1
  • Sulfotransferase 2B1
  • sulfotransferase family, cytosolic, 2B, member 1
  • SULT2B1

Background

SULT2B1 (Sulfotransferase 2B1; also ST2B1b and alcohol sulfotransferase) is a 45-48 kDa member of the sulfotransferase 1 family of enzymes. SULT2B1 is widely expressed, being found in platelets, stratum granulosum keratinocytes, breast and prostatic epithelium, and syncytiotrophoblast cells. SULT2B1 catalyzes the sulfonation of DHEA, a precursor for sex steroids, and cholesterol, which supports stratification of the epidermis. Human SULT2B1 is 365 amino acids (aa) in length. There are PAPS binding sites between aa 70-75 and 147-155, a myristoylation motif between aa 255-259, and a Pro-rich region between aa 305-364 that may extend enzyme half-life. There is one 43 kDa splice form (ST2B1a) that shows an eight aa substitution for aa 1-23. It is expressed in fetal brain and generates pregnenolone sulfate, a steroid that modulates neurotransmitters. Full-length human SULT2B1b shares only 71% aa identity with mouse SULT2B1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytosolic Sulfotransferase 2B1/SULT2B1 Antibody (AF6174)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol SULT2B1
Uniprot