TRIM37 Antibody - BSA Free

Western Blot: TRIM37 Antibody [NBP2-20715] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: NCI-H929 7. 5% SDS PAGE gel, diluted at 1:1000.

Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM37 antibody [N1N2], N-term (NBP2-20715) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TRIM37 antibody [N1N2], N-term (NBP2-20715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

• Reactivity: Hu, Rt, Mu, Bv, Ch, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TRIM37 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TRIM37 Antibody - BSA Free (NBP2-20715) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human TRIM37. The exact sequence is proprietary.
• Localization: Cytoplasm; Peroxisome
• Predicted Species: Mouse (99%), Rhesus Macaque (99%), Bovine (98%), Chicken (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TRIM37
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 108 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TRIM37 Antibody - BSA Free

• EC 6.3.2.-
• KIAA0898Tripartite motif-containing protein 37
• Mulibrey nanism protein
• MULRING-B-box-coiled-coil protein
• POB1E3 ubiquitin-protein ligase TRIM37
• TEF3
• tripartite motif containing 37
• tripartite motif-containing 37

Background

This gene encodes a member of the tripartite motif (TRIM) family, whose members are involved in diverse cellular functions such as developmental patterning and oncogenesis. The TRIM motif includes zinc-binding domains, a RING finger region, a B-box motif and a coiled-coil domain. The RING finger and B-box domains chelate zinc and might be involved in protein-protein and/or protein-nucleic acid interactions. The gene mutations are associated with mulibrey (muscle-liver-brain-eye) nanism, an autosomal recessive disorder that involves several tissues of mesodermal origin. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: TRIM37
• Uniprot:
  • O94972(Human)