TRIM27 Antibody (PCRP-TRIM27-1B3)

Immunocytochemistry/Immunofluorescence: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415] - Immunofluorescence Analysis of PFA-fixed U87 cells using TRIM27 Mouse Monoclonal Antibody (PCRP-TRIM27-1B3) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Flow Cytometry: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TRIM27 Mouse Monoclonal Antibody (PCRP-TRIM27-1B3) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Protein Array: TRIM27 Antibody (PCRP-TRIM27-1B3) [NBP3-07415] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TRIM27 Mouse Monoclonal Antibody (PCRP-TRIM27-1B3).

• Reactivity: Hu
• Applications: Flow, ICC/IF, MA
• Clone: PCRP-TRIM27-1B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TRIM27 Antibody (PCRP-TRIM27-1B3) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08579)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human TRIM27 protein (Uniprot: P14373)
• Localization: Nucleus, Cytoplasm. Nucleus > PML body. Nuclear or cytoplasmic depending on the cell type (By similarity). Colocalized with PML and EIF3S6 in nuclear bodies.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM27
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 58.49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for TRIM27 Antibody (PCRP-TRIM27-1B3)

• Ret finger proteinRING finger protein 76
• RFPtripartite motif protein TRIM27
• RNF76RFP transforming protein
• tripartite motif containing 27
• tripartite motif-containing 27
• Tripartite motif-containing protein 27
• zinc finger protein RFP

Background

The TRIM27 gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM27
• Uniprot:
  • P14373(Human)