TRIM Antibody (488806)

Human blood‑derived CD3+ lymphocytes were stained with Mouse Anti-Human TRIM Monoclonal Antibody (Catalog # MAB4708, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intra­cellular staining, cells were fixed with paraformaldehyde and permea­bilized with saponin.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clone: 488806
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TRIM Antibody (488806) Summary

• Immunogen: E. coli-derived recombinant human TRIM
  Thr50-Asn186
  Accession # Q6PIZ9
• Specificity: Detects human TRIM in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRAT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRIM Antibody (488806)

• pp29/30
• T cell receptor associated transmembrane adaptor 1
• T cell receptor interacting molecule
• T-cell receptor interacting molecule
• T-cell receptor-associated transmembrane adapter 1
• T-cell receptor-interacting molecule
• TCRIM
• TRAT1
• TRIM
• TRIMHSPC062

Background

Human TRIM (T cell receptor-interacting molecule) (also trat1, T cell receptor-associated transmembrane adaptor 1, and pp29/30) is a 30 kDa, type III transmembrane protein, that is a member of the transmembrane adaptor protein (TRAP) family. It contains a short, 8 aa extracellular region, a 19 aa transmembrane region, and a 159 aa cytosolic tail. Its cytoplasmic tail contains several tyrosine motifs with the potential to bind to Src-homology 2 (SH2) domains of signaling proteins. TRIM is present in T cells and NK cells. Human TRIM shares 66% aa sequence identity with mouse TRIM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAT1
• Uniprot:
  • Q6PIZ9()