Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free

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Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready
Clone
34BE12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1.0 mg/ml

Order Details

Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free Summary

Immunogen
Solubilized keratin extract from human stratum corneum
Localization
Cytoplasmic
Marker
Epithelial Marker
Specificity
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
KRT76
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1.0ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1.0ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • CyTOF-ready
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1.0 mg/ml
Purity
Protein A purified

Alternate Names for Cytokeratin, HMW Antibody (34BE12) - Azide and BSA Free

  • CK-2P
  • cytokeratin 2
  • Cytokeratin-2P
  • HUMCYT2A
  • K2P
  • K76
  • keratin 76
  • keratin, type II cytoskeletal 2 oral
  • keratin-76
  • KRT2Bcytokeratin-2P
  • KRT2Pkeratin 2p
  • KRT76
  • Type-II keratin Kb9

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin, HMW Antibody (NBP2-34533) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34533AF350
Alexa Fluor 405 NBP2-34533AF405
Alexa Fluor 488 NBP2-34533AF488
Alexa Fluor 532 NBP2-34533AF532
Alexa Fluor 594 NBP2-34533AF594
Alexa Fluor 647 NBP2-34533AF647
Alexa Fluor 700 NBP2-34533AF700
Alexa Fluor 750 NBP2-34533AF750
Allophycocyanin NBP2-34533APC
Biotin NBP2-34533B
DyLight 350 NBP2-34533UV
DyLight 405 NBP2-34533V
DyLight 488 NBP2-34533G
DyLight 550 NBP2-34533R
DyLight 594 NBP2-34533DL594
DyLight 650 NBP2-34533C
DyLight 680 NBP2-34533FR
DyLight 755 NBP2-34533IR
HRP NBP2-34533H
Janelia Fluor 549 NBP2-34533JF549
Janelia Fluor 646 NBP2-34533JF646
PE NBP2-34533PE

Additional Cytokeratin, HMW Products

Bioinformatics Tool for Cytokeratin, HMW Antibody (NBP2-34533)

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Bioinformatics

Gene Symbol KRT76