TRAP1 Antibody (OTI1H8)

Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] Analysis of extracts (35ug) from 9 different cell lines by using anti-TRAP1 monoclonal antibody.

Immunocytochemistry/Immunofluorescence: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY TRAP1.

Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Human Kidney tissue using anti-TRAP1 mouse monoclonal antibody.

Flow Cytometry: TRAP1 Antibody (1H8) [NBP2-01707] - Analysis of Jurkat cells, using anti-TRAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Western Blot: TRAP1 Antibody (1H8) [NBP2-01707] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRAP1.

Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-TRAP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: TRAP1 Antibody (1H8) [NBP2-01707] - Staining of paraffin-embedded Human endometrium tissue using anti-TRAP1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC
• Clone: OTI1H8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TRAP1 Antibody (OTI1H8) Summary

• Immunogen: Full length human recombinant protein of human TRAP1(NP_057376) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:500-2000
• Theoretical MW: 79.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRAP1 Antibody (OTI1H8)

• HSP 75
• HSP75TNFR-associated protein 1
• HSP90Lheat shock protein 75 kDa, mitochondrial
• TNF receptor-associated protein 1
• TRAP-1
• tumor necrosis factor type 1 receptor associated protein
• Tumor necrosis factor type 1 receptor-associated protein

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. TRAP1 is a mitochondrial HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1; MIM 140571) and endoplasmic reticulum (HSP90B1; MIM 191175) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAP1
• Entrez:
  • 10131(Human)