Transglutaminase 4/TGM4 Antibody

Western Blot: Transglutaminase 4 Antibody [NBP1-97776] - (1ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Transglutaminase 4/TGM4 Antibody Summary

• Immunogen: Peptide with sequence C-NQPLQSYHQLKLEFS corresponding to internal region (near N-Terminus) according to NP_003232.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TGM4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in HeLa lysates (calculated MW of 77.1 kDa band according to NP_003232.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Transglutaminase 4/TGM4 Antibody

• EC 2.3.2.13
• Fibrinoligase
• hTGP
• Prostate transglutaminase
• Prostate-specific transglutaminase
• protein-glutamine gamma-glutamyltransferase 4
• TG(P)
• TGase P
• TGase-4
• TGM4
• TGP
• TGPFLJ26776
• transglutaminase 4 (prostate)
• Transglutaminase 4
• Transglutaminase P
• transglutaminase-4

Background

TGM4 (Transglutaminase 4; also TGP and Prostate-specific transglutaminase) is a 77kDa member of the papain-like family of transglutaminases. TGM4 is a unique member of the family due largely to its distribution pattern in the body in that it is primarily found in the prostate gland and existed only at low levels in other tissues types.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transglutaminase 4/TGM4 Antibody (NBP1-97776)(1)

Publication using NBP1-97776

• Jiang WG, Ye L, Ablin RJ et al. The prostate transglutaminase, TGase-4, coordinates with the HGFL/MSP RON system in stimulating the migration of prostate cancer cells. Int J Oncol 2010-08-01 [PMID: 20596668]

Bioinformatics

• Gene Symbol: TGM4
• Entrez:
  • 7047(Human)
• Uniprot:
  • NP_003232.2(Human)