Transglutaminase 4/TGM4 Antibody [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 750

Transglutaminase 4/TGM4 Antibody [Alexa Fluor® 750] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Transglutaminase 4
  Met2-Lys684
  Accession # AAC50516
• Specificity: Detects human Transglutaminase 4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Transglutaminase 2, rhTransglutaminase 3, rhTransglutaminase 7, and recombinant m
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Transglutaminase 4/TGM4 Antibody [Alexa Fluor® 750]

• EC 2.3.2.13
• Fibrinoligase
• hTGP
• Prostate transglutaminase
• Prostate-specific transglutaminase
• protein-glutamine gamma-glutamyltransferase 4
• TG(P)
• TGase P
• TGase-4
• TGM4
• TGP
• TGPFLJ26776
• transglutaminase 4 (prostate)
• Transglutaminase 4
• Transglutaminase P
• transglutaminase-4

Background

TGM4 (Transglutaminase 4; also TGP and Prostate-specific transglutaminase) is a 77 kDa member of the papain-like family of transglutaminases. It is expressed by various tumor cell lines plus prostatic epithelium, and may participate in semen protein modification. TGM4, like other TGMs, can apparently mediate transamidation, plus esterification reactions. Human TGM4 is 684 amino acids (aa) in length. Although it does not contain a signal sequence, based on rodent, it is likely secreted as a homodimer. TGM4 contains an active enzyme site between aa 263‑353, and shows a C-terminal Ig-like domain (aa 587‑684). The molecule has a requirement for divalent calcium. There are two potential splice variants. One shows a 45 aa insertion after Leu8, while another shows a 12 aa substitution for aa 1‑80. Human full‑length TGM4 shares only 54% aa identity with mouse TGM4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.