IL-24 Antibody [Unconjugated]

Recombinant Human IL‑24 (Catalog # 1965-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑24 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1965). The ND50 is typically < 0.6 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

IL-24 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑24
  Gln50-Leu206
  Accession # Q2YHE5
• Specificity: Detects human IL-24 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL24
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Neutralization 0.2-0.6 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-24 Antibody [Unconjugated]

• C49A
• FISP
• IL10B
• IL24
• IL-24
• interleukin 24
• MDA-7
• MDA7MOB5
• melanocyte-associated Mda-7
• melanoma differentiation association protein 7
• Melanoma differentiation-associated gene 7 protein
• MOB-5
• ST16
• ST16interleukin-24
• suppression of tumorigenicity 16 (melanoma differentiation)
• Suppression of tumorigenicity 16 protein

Background

Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids that contains a 48 amino acid (aa) signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35-40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4⁺ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma , IL-1 beta , IL‑12, and TNF-alpha . At high concentrations, it is a strong inducer of apoptosis in tumor cells but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta , which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta , which is shared with IL-20.

1. Jiang, H. et al. (1995) Oncogene 11:2477.
2. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160.
3. Wang, M. et al. (2002) J. Biol Chem. 227:7341.
4. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649.
5. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
6. Chen, J. et al. (2003) Molec. Ther. 8:220.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-24 Antibody (AF1965)(15)

Publications using AF1965

• SC Chang, BX Zhang, EC Su, WC Wu, TH Hsieh, AM Salazar, YK Lin, JL Ding Hiltonol Cocktail Kills Lung Cancer Cells by Activating Cancer-Suppressors, PKR/OAS, and Restraining the Tumor Microenvironment International Journal of Molecular Sciences, 2021-02-05;22(4):. 2021-02-05 [PMID: 33562773] (Neutralization, Human)
• Q Mo, L Liu, G Bao, T Li Effects of melanoma differentiation associated gene-7 (MDA-7/IL-24) on apoptosis of liver cancer cells via regulating the expression of B-cell lymphoma-2 Oncol Lett, 2019-05-02;18(1):29-34. 2019-05-02 [PMID: 31289468] (Western Blot, Human)
• Jacek Lubkowski, Cem Sonmez, Sergey V. Smirnov, Andriy Anishkin, Sergei V. Kotenko, Alexander Wlodawer Crystal structure of the labile complex of interleukin 24 (IL-24) with the extracellular domains of IL-22R1 and IL-20R2 The Journal of Immunology 10/1/2018 [PMID: 30111632]
• Erin E. Olsan, Jonathan D. West, Jacob A. Torres, Nicholas Doerr, Thomas Weimbs Identification of targets of IL-13 and STAT6 signaling in polycystic kidney disease American Journal of Physiology-Renal Physiology 7/1/2018 [PMID: 29513071]
• B Liu, F Chen, Y Wu, X Wang, M Feng, Z Li, M Zhou, Y Wang, L Wu, X Liu, D Liang Enhanced tumor growth inhibition by mesenchymal stem cells derived from iPSCs with targeted integration of interleukin24 into rDNA loci Oncotarget, 2017-06-20;8(25):40791-40803. 2017-06-20 [PMID: 28388559] (ELISA Development, IHC, Western Blot, Human, Mouse)
• QUNFENG MA, BANGMING JIN, YAO ZHANG, YINAN SHI, CHI ZHANG, DAN LUO et al. Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo Oncology Reports 5/1/2016 [PMID: 26935394]
• QUNFENG MA, BANGMING JIN, YAO ZHANG, YINAN SHI, CHI ZHANG, DAN LUO et al. Secreted recombinant human IL-24 protein inhibits the proliferation of esophageal squamous cell carcinoma Eca-109 cells in vitro and in vivo Oncology Reports 2016-05-01 [PMID: 26935394] (Neutralization, Immunocytochemistry, Human)
• ZONGMING LIU, LIBO XU, HONGPING YUAN, YANG ZHANG, XIAONA ZHANG, DONGXU ZHAO Oncolytic adenovirus-mediated mda-7/IL-24 expression suppresses osteosarcoma growth and enhances sensitivity to doxorubicin Molecular Medicine Reports 10/1/2015 [PMID: 26251997]
• QUNFENG MA, XUEFENG DENG, BANGMING JIN, YAO ZHANG, DAN LUO, HEYU SONG et al. A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells Oncology Reports 1/1/2015 [PMID: 25371158]
• QUNFENG MA, XUEFENG DENG, BANGMING JIN, YAO ZHANG, DAN LUO, HEYU SONG et al. A novel human interleukin-24 peptide created by computer-guided design contributes to suppression of proliferation in esophageal squamous cell carcinoma Eca-109 cells Oncology Reports 2015-01-01 [PMID: 25371158] (Immunocytochemistry, Human)
• Anuradha R, George P, Hanna L, Kumaran P, Chandrasekaran V, Nutman T, Babu S Expansion of parasite-specific CD4+ and CD8+ T cells expressing IL-10 superfamily cytokine members and their regulation in human lymphatic filariasis. PLoS Negl Trop Dis, 2014-04-03;8(4):e2762. 2014-04-03 [PMID: 24699268] (Flow Cytometry, Human)
• Shefler I, Pasmanik-Chor M, Kidron D, Mekori Y, Hershko A T cell-derived microvesicles induce mast cell production of IL-24: relevance to inflammatory skin diseases. J Allergy Clin Immunol, 2013-06-12;133(1):217-24.e1-3. 2013-06-12 [PMID: 23768573] (Neutralization, Human)
• Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C Induction of phenotype modifying cytokines by FERMT1 mutations. Hum. Mutat., 2011-03-01;32(4):397-406. 2011-03-01 [PMID: 21309038] (Neutralization, Human)
• Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells. Blood, 2009-11-24;115(9):1718-26. 2009-11-24 [PMID: 19965640] (IHC-Fr, Human)
• Tahara I, Miyake K, Hanawa H, Kurai T, Hirai Y, Ishizaki M, Uchida E, Tajiri T, Shimada T Systemic cancer gene therapy using adeno-associated virus type 1 vector expressing MDA-7/IL24. Mol. Ther., 2007-06-05;15(10):1805-11. 2007-06-05 [PMID: 17551500] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: IL24
• Uniprot:
  • Q2YHE5()