Transferrin Antibody [Unconjugated]

Western blot shows purified holo-transferrin, purified apo-transferrin, and human plasma. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Transferrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Transferrin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Transferrin was detected in perfusion fixed frozen sections of mouse thymus using Goat Anti-Mouse Transferrin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3987) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to the plasma membranes of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Transferrin Antibody [Unconjugated] Summary

• Immunogen: Mouse plasma-derived Transferrin
• Specificity: Detects mouse Transferrin in direct ELISAs. Detects human Transferrin in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TF
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Transferrin Antibody [Unconjugated]

• Beta-1 metal-binding globulin
• DKFZp781D0156
• EC 3.4.21
• PRO1557
• PRO2086
• Serotransferrin
• Siderophilin
• TF
• Transferrin

Background

Transferrin (also serotransferrin and siderophilin) is a secreted, monomeric 78-82 kDa glycoprotein member of the transferrin family of molecules. It is synthesized by hepatocytes and serves as a transport vehicle for ferric iron, as well as cobalt and manganese ions. When bound to iron, transferrin is referred to as holo-transferrin (Greek: holo - meaning whole or together). When it is absent iron, it is called apotransferrin (Greek: apo - meaning away or apart). Apotransferrin encounters and binds two ferric iron atoms at the basolateral surface of duodenal epithelium. Here, as holo-transferrin, it circulates and distributes iron to virtually all tissues by binding to transferrin receptor 1. Once bound, holo-transferrin is internalized, iron is released, and the resulting apotransferrin is recylced. Mature mouse apotransferrin is 678 amino acids (aa) in length. It is bilobar in shape, with 330 aa N- and C-terminal lobes that each bind one ferric atom. In the absence of iron, each lobe is "open"; when iron is present, the lobes close, forming a compact structure. Mature mouse apotransferrin is 72% and 88% aa identical to human and rat apotransferrin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TF