Transferrin Antibody (TF/3001)

Immunohistochemistry-Paraffin: Transferrin Antibody (TF/3001) [NBP2-79778] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Transferrin Antibody (TF/3001).

Protein Array: Transferrin Antibody (TF/3001) [NBP2-79778] - Analysis of Protein Array containing >19,000 full-length human proteins using Transferrin Antibody (TF/3001) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

• Reactivity: Hu
• Applications: ELISA, IHC, MA
• Clone: TF/3001
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Transferrin Antibody (TF/3001) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-79912)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Recombinant fragment (around aa 311-445) of human Transferrin protein (exact sequence is proprietary) (Uniprot: P02787)
• Localization: Secreted
• Marker: Early Marker of Oligodendrocytes
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TF
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA 2-4 ug/ml
  • Immunohistochemistry 1-2 ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
• Application Notes: ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
  Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 79 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Transferrin Antibody (TF/3001)

• Beta-1 metal-binding globulin
• DKFZp781D0156
• EC 3.4.21
• PRO1557
• PRO2086
• Serotransferrin
• Siderophilin
• TF
• Transferrin

Background

Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TF
• Uniprot:
  • P02787(Human)