Recombinant Human TRANCE/TNFSF11/RANK L Animal-Free Protein

Images

 
Bioactivity: Recombinant Human TRANCE/TNFSF11/RANK L Animal-Free Protein [NBP2-76190] - TRANCE/TNFSF11/RANK L induced IL-8 production by human PBMCs.
SDS-Page: Recombinant Human TRANCE/TNFSF11/RANK L Animal-Free Protein [NBP2-76190] - 1 ug in each lane (-) non-reducing conditions and (+) reducing conditions in a 4-20% Tris-Glycine gel.
Bioactivity: Recombinant Human TRANCE/TNFSF11/RANK L Animal-Free Protein [NBP2-76190] - TRANCE/TNFSF11/RANK L induced SEAP production by RAW-blue (TM) cells.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE, Bioactivity
Format
Animal Free
Concentration
LYOPH

Order Details

View Available Formulations

Recombinant Human TRANCE/TNFSF11/RANK L Animal-Free Protein Summary

Description
An un-tagged bioactive recombinant protein corresponding to Human TRANCE/TNFSF11/RANK L

Source: E. coli

Amino Acid Sequence: EKAMVDGSWL DLAKRSKLEA QPFAHLTINA TDIPSGSHKV SLSSWYHDRG WAKISNMTFS NGKLIVNQDG FYYLYANICF RHHETSGDLA TEYLQLMVYV TKTSIKIPSS HTLMKGGSTK YWSGNSEFHF YSINVGGFFK LRSGEEISIE VSNPSLLDPD QDATYFGAFK VRDID

Produced with no materials of animal or human origin using ANIMAL-FREE purification processes.

Production and Purification

  • No animal-derived ingredients, No FBS
  • E. coli based production system
  • Animal-Free facility, fermentor, columns, filters, labware, lyophilization, and storage

Quality Assurance

  • Endotoxin levels at <1 EUs/ug
  • Purity levels at 95% or greater
Specificity
TRANCE/TNFSF11/RANK L is a monomer, 19.7 kDa (175 aa)
Preparation
Method
Protein is derived from genetically modified E. coli.
Details of Functionality
Activity of RAW-Blue(TM) cells, >2.0 x 10^4 units/mg (ED50 acceptance criteria are <50 ng/mL).
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
TNFSF11
Purity
>95%, by SDS-PAGE
Endotoxin Note
Acceptance criteria for endotoxin levels are <1 EUs/ug protein (determined by Kinetic LAL Method).

Applications/Dilutions

Dilutions
  • Bioactivity
  • SDS-Page
Theoretical MW
19.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Preservative
No Preservative
Concentration
LYOPH
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Sterile water at 0.1 mg/mL

Notes

Lot Specific Information:
Given endotoxin and ED50 values are acceptance criteria standards. For lot specific values, please contact technical support.

Detailed Reconstitution:
Centrifuge vial before opening. Suspend the product by gently pipetting the recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots.

Expiration Date:
12 months from date of receipt when stored at -20C to-80C as supplied. 1 month when stored at 4C after reconstituting as directed. 3 months when stored at -20C to -80C after reconstituting as directed.

Alternate Names for Recombinant Human TRANCE/TNFSF11/RANK L Animal-Free Protein

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • OPGLOPTB2
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKL
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • TRANCE
  • TRANCEODFhRANKL2
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11

Background

TRANCE/OPGL/RANKL/ODF is a recently identified member of tumor necrosis factor family. TRANCE (TNF-related activation-induced cytokine receptor) or RANKL {receptor activator of NF-kB (RANK) ligand} has been implicated in interactions between T cells and dendritic cells (1-2). RANK ligand (RANKL/TRANCE) binds to RANK on dendritic cells, upregulates the expression of anti-apoptotic protein BcL-XL suggesting a role in dendritic cell survival (2). TRANCE/RANKL is also important in T and B-cell maturation (3). As OPGL/ODF (Osteoprotegerin Ligand/Osteoclast differentiation factor), the same protein can both activate mature osteoclasts and mediate osteoclastogenesis (4). OPGL/TRANCE deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56508
Species: Av, Hu, Mu, Rt
Applications: CyTOF-ready, Flow-CS, Flow, IHC, IHC-P, WB
DY805
Species: Hu
Applications: ELISA
210-TA
Species: Hu
Applications: BA
D6050
Species: Hu
Applications: ELISA
DCDL40
Species: Hu
Applications: ELISA
NB100-56173
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
MAB1419
Species: Hu, Rt
Applications: CyTOF-ready, ICC, IHC, ICFlow
H00000054-D01P
Species: Hu, Mu
Applications: IHC, IHC-P, WB
H00057410-M02
Species: Hu, Mu, Rt
Applications: ELISA, S-ELISA, WB
NBP1-87315
Species: Hu
Applications: IHC, IHC-P
MAB7665
Species: Hu
Applications: IHC, WB
H00001513-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB100-56583
Species: Hu, Rb, Rt
Applications: Flow, IHC, IHC-P, KO, WB
NB100-56732
Species: Hu, Mu
Applications: ICC/IF, IHC, Simple Western, WB
NBP1-47842
Species: Ca, Hu, Pm, Mu, Rt
Applications: IHC, IHC-P, IP, WB
NBP2-50037
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, KO, WB
NB300-164
Species: Ca, Hu, Mu, Po, Pm, Rt(-)
Applications: Dual ISH-IHC, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, WB
NBP2-76190
Species: Hu
Applications: PAGE, Bioactivity

Publications for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190) (0)

There are no publications for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190) (0)

There are no reviews for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TRANCE/TNFSF11/RANK L Products

Bioinformatics Tool for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190)

Discover related pathways, diseases and genes to TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190)

Discover more about diseases related to TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190).
 

Pathways for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190)

View related products by pathway.

PTMs for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190)

Learn more about PTMs related to TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190).
 

Research Areas for TRANCE/TNFSF11/RANK L Recombinant Protein (NBP2-76190)

Find related products by research area.

Blogs on TRANCE/TNFSF11/RANK L.

The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human TRANCE/TNFSF11/RANK L Animal-Free Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFSF11