Recombinant Mouse TRANCE/TNFSF11/RANK L Protein

Product Discontinued

Recombinant Mouse TRANCE/TNFSF11/RANK L Protein Summary

• Description: A biologically active protein to TNFSF11.Source: E. coli
• Preparation Method: Lyophilized from 0.2 mm filtered concentrated solution in 20 mM PBS, pH 7.0, 200 mM NaCl, 0.1 mM EDTA.
• Details of Functionality: The ED50, as determined by inducing osteoclast differentiation using murine RAW 264.7 cells, is less than 5 ng/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: TNFSF11
• Purity: SDS-PAGE
• Endotoxin Note: Less than 1 EU/mg of the rMuTRANCE/RANK L/TNFSF11 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • Functional
  • SDS-Page
• Application Notes: Novus Biologicals' NovActive proteins are fully active and stringently purified to provide only the safest and highest efficacy proteins available. NovActive proteins are expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance. NovActive proteins are guaranteed to be greater than 90% pure by HPLC, and contain less than 0.005 EU/ug of endotoxin.
• Theoretical MW: 19 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Purity: SDS-PAGE
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with sterilized PBS or aqueous buffer to a final concentration of 0.1 mg/ml.

Alternate Names for Recombinant Mouse TRANCE/TNFSF11/RANK L Protein

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

RANK Ligand (RANK L), also known as TRANCE, TNFSF11, OPGL, and ODF, is an approximately 40 kDa transmembrane protein in the TNF cytokine superfamily. RANK L associates into trimers, and multiple soluble forms can be generated by ADAM10, MMP14, or MT1-MMP mediated cleavage. Both transmembrane and soluble RANK L act through RANK receptors and are antagonized by the decoy receptor, Osteoprotegrin. Within the ECD, human and mouse RANK L share 85% aa sequence identity. RANK L is produced by osteoblasts, bone marrow stromal cells, activated T cells, mammary epithelial cells, and astrocytes. It is promotes osteoclast differentiation, dendritic cell activation, mammary gland function, and body temperature regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF11