TRANCE/TNFSF11/RANK L Antibody (70525) [Unconjugated]

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In the presence of Recombinant Mouse M‑CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in RAW 264.7 mouse macrophages in a ...read more
TRANCE/TNFSF11/RANK L was detected in immersion fixed paraffin-embedded sections of human lymph node using 5 µg/mL Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB626) overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, B/N
Clone
70525
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

TRANCE/TNFSF11/RANK L Antibody (70525) [Unconjugated] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human TRANCE/TNFSF11/RANK L
Gly136-Asp317 (Ala194Gly)
Accession # O14788
Specificity
Detects human TRANCE/TNFSF11/RANK L in direct ELISAs. No cross-reactivity with recombinant mouse TRANCE is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
TNFSF11
Purity Statement
Protein A or G purified from ascites
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 0.8-2.5 ug/mL
Publications
Read Publications using
MAB626 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (70525) [Unconjugated]

  • CD254 antigen
  • CD254
  • ODF
  • OPGL
  • OPGLOPTB2
  • Osteoclast differentiation factor
  • Osteoprotegerin ligand
  • RANK L
  • RANKL
  • RANKLreceptor activator of nuclear factor kappa B ligand
  • Receptor activator of nuclear factor kappa-B ligand
  • sOdf
  • TNF-related activation-induced cytokine
  • TNFSF11
  • TRANCE
  • TRANCEODFhRANKL2
  • tumor necrosis factor (ligand) superfamily, member 11
  • tumor necrosis factor ligand superfamily member 11

Background

TNF-related activation-induced cytokine (TRANCE; also RANKL, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family. Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation. Splicing variants produce three isoforms for human TRANCE. Isoform 1 is the standard form. In isoform 2 lacks aa 1-73, and isoform 3 lacks aa 1-47. TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart,placenta, skeletal muscle, stomach, and thyroid. TRANCE plays a role in osteoclast differentiation and activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRANCE/TNFSF11/RANK L Antibody (MAB626)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(2)
Neutralization
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(8)
All Species
Showing Publications 1 - 8 of 8.
Publications using MAB626 Applications Species
I Gomes, BP de Almeida, S Dâmaso, A Mansinho, I Correia, S Henriques, R Cruz-Duart, G Vilhais, P Félix, P Alves, P Corredeira, NL Barbosa-Mo, L Costa, S Casimiro Expression of receptor activator of NFkB (RANK) drives stemness and resistance to therapy in ER+HER2- breast cancer Oncotarget, 2020;11(19):1714-1728. 2020 [PMID: 32477461] (Neutralization, Human) Neutralization Human
CGJ Moonen, TJ de Vries, P Rijkschroe, PE Poubelle, EA Nicu, BG Loos The Possible Role of Neutrophils in the Induction of Osteoclastogenesis J Immunol Res, 2019;2019(0):8672604. 2019 [PMID: 31637266] (ICC, Human) ICC Human
Amiable N, Tat SK, Lajeunesse D, Duval N, Pelletier JP, Martel-Pelletier J, Boileau C Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts. Bone, 2009;44(6):1143-50. 2009 [PMID: 19264156] (Flow Cytometry, Human) Flow Cytometry Human
Gendron S, Boisvert M, Chetoui N, Aoudjit F alpha1beta1 integrin and interleukin-7 receptor up-regulate the expression of RANKL in human T cells and enhance their osteoclastogenic function. Immunology, 2008;125(0):359. 2008 [PMID: 18479350] (Western Blot, Human) Western Blot Human
Lee N, Smolarz AJ, Olson S, David O, Reiser J, Kutner R, Daw NC, Prockop DJ, Horwitz EM, Gregory CA A potential role for Dkk-1 in the pathogenesis of osteosarcoma predicts novel diagnostic and treatment strategies. Br. J. Cancer, 2007;97(11):1552-9. 2007 [PMID: 17987039] (Western Blot, Human) Western Blot Human
Makrygiannakis D, af Klint E, Catrina SB, Botusan IR, Klareskog E, Klareskog L, Ulfgren AK, Catrina AI Intraarticular corticosteroids decrease synovial RANKL expression in inflammatory arthritis. Arthritis Rheum., 2006;54(5):1463-72. 2006 [PMID: 16646024] (Western Blot, Human) Western Blot Human
Fonseca JE, Cortez-Dias N, Francisco A, Sobral M, Canhao H, Resende C, Castelao W, Macieira C, Sequeira G, Saraiva F, da Silva JA, Carmo-Fonseca M, Viana Queiroz M Inflammatory cell infiltrate and RANKL/OPG expression in rheumatoid synovium: comparison with other inflammatory arthropathies and correlation with outcome. Clin. Exp. Rheumatol., 2005;23(2):185-92. 2005 [PMID: 15895888] (IHC, Human) IHC Human
Mandelin J, Li TF, Hukkanen M, Liljestrom M, Salo J, Santavirta S, Konttinen YT Interface tissue fibroblasts from loose total hip replacement prosthesis produce receptor activator of nuclear factor-kappaB ligand, osteoprotegerin, and cathepsin K. J. Rheumatol., 2005;32(4):713-20. 2005 [PMID: 15801030] (IHC, Human) IHC Human

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Bioinformatics Tool for TRANCE/TNFSF11/RANK L Antibody (MAB626)

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Diseases for TRANCE/TNFSF11/RANK L Antibody (MAB626)

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Blogs on TRANCE/TNFSF11/RANK L.

The diverse functions of RANKL/TRANCE/TNFSF11
RANKL (also known as TNF-related activation-induced cytokine), or receptor activator of nuclear factor-?B ligand, was first discovered as a key player in the RANKL/RANK/OPG osteoclast formation pathway. Osteoclasts are large multinucleate cells t...  Read full blog post.

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Bioinformatics

Gene Symbol TNFSF11
Uniprot