TRANCE/TNFSF11/RANK L Antibody (685857) [Allophycocyanin]

Human peripheral blood mononuclear cells (PBMCs) treated with PHA for were stained with Mouse Anti-Human IL-2 Ra PE-conjugated Monoclonal Antibody (Catalog # FAB1020P) and either (A) Mouse Anti-Human TRANCE/TNFSF11/RANK L APC-conjugated Monoclonal Antibody (Catalog # FAB6264A) or (B) Mouse IgG2BAllophycocyanin Isotype Control (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Human HEK293 cell line transfected with human TRANCE was stained with Mouse Anti-Human TRANCE/TNFSF11/RANK L APC-conjugated Monoclonal Antibody (Catalog # FAB6264A, filled histogram) or Mouse IgG2B Allophycocyanin Isotype Control (Catalog # IC0041A, open histogram).View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 685857
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

TRANCE/TNFSF11/RANK L Antibody (685857) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
  Gly64-Asp245
  Accession # AAC51762
• Specificity: Detects human TRANCE/TNFSF11/RANK L in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (685857) [Allophycocyanin]

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

TNF-related activation-induced cytokine (TRANCE; also RANK L, OPGL, and ODF) is a 35 kDa (predicted) type II transmembrane glycoprotein and member of the TNF cytokine family.  Human TRANCE is 317 amino acids (aa) in length and contains a 47 aa cytoplasmic region, a 21 aa transmembrane region, and a 249 extracellular domain (ECD), which contains two potential sites of N-linked glycosylation.  Splicing variants produce three isoforms for human TRANCE.  Isoform 1 is the standard form.  In isoform 2, aa corresponding to 1-73 in isoform 1 are missing.  In isoform 3, aa 1-47 are missing.  Human TRANCE ECD is 83% identical to mouse TRANCE ECD.  TRANCE is expressed highest in the peripheral lymph nodes and weaker in the spleen, peripheral blood leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach, and thyroid.  TRANCE plays a role in osteoclast differentiation and activation. 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (FAB6264A)(1)

Publication using FAB6264A

• Qi-Wen Man, Wen-Qun Zhong, Jian-Gang Ren, Jin-Yuan Liu, Yue-Yu Zheng, Rui-Fang Li et al. Increased level of cell-derived microparticles in the cyst fluids of odontogenic keratocysts International Journal of Oncology 2018-04-05 [PMID: 29620170]

Bioinformatics

• Gene Symbol: TNFSF11
• Uniprot:
  • AAC51762()