TRANCE/TNFSF11/RANK L Antibody (175707) [Unconjugated]

In the presence of Recombinant Mouse M‑CSF (20 ng/mL, Catalog # 416-ML), Recombinant Human TRANCE/TNFSF11/RANK L (Catalog # 390-TN) induces osteoclast-like cell formation in the RAW 264.7 mouse monocyte/macrophage cell line in a dose-dependent manner (orange line), as measured by TRAP (tartrate-resistant acid phosphatase) activity in cell lysates . Under these conditions, osteoclast-like cell formation elicited by Recombinant Human TRANCE/TNFSF11/RANK L (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human TRANCE/TNFSF11/RANK L Monoclonal Antibody (Catalog # MAB6262). The ND50 is typically 0.01‑0.04 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 175707
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TRANCE/TNFSF11/RANK L Antibody (175707) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TRANCE/TNFSF11/RANK L
  Gly64-Asp245
  Accession # AAC51762
• Specificity: Detects human TRANCE/TNFSF11/RANK L in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF11
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.01-0.04 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRANCE/TNFSF11/RANK L Antibody (175707) [Unconjugated]

• CD254 antigen
• CD254
• ODF
• OPGL
• OPGLOPTB2
• Osteoclast differentiation factor
• Osteoprotegerin ligand
• RANK L
• RANKL
• RANKLreceptor activator of nuclear factor kappa B ligand
• Receptor activator of nuclear factor kappa-B ligand
• sOdf
• TNF-related activation-induced cytokine
• TNFSF11
• TRANCE
• TRANCEODFhRANKL2
• tumor necrosis factor (ligand) superfamily, member 11
• tumor necrosis factor ligand superfamily member 11

Background

TRANCE, also known as RANK ligand, is a type II membrane protein belonging to the tumor necrosis factor superfamily and has been designated TNFSF11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRANCE/TNFSF11/RANK L Antibody (MAB6262)(1)

Publication using MAB6262

• Barbaroux JB, Beleut M, Brisken C, Mueller CG, Groves RW Epidermal receptor activator of NF-kappaB ligand controls Langerhans cells numbers and proliferation. J. Immunol., 2008-07-15;181(2):1103-8. 2008-07-15 [PMID: 18606662] (Flow Cytometry, ICC, Human)

Bioinformatics

• Gene Symbol: TNFSF11
• Uniprot:
  • AAC51762()