TRAIP Antibody

Immunohistochemistry-Paraffin: TRAIP Antibody [NB100-1230] - Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRAIP Antibody Summary

• Immunogen: Peptide with sequence C-SLFQAKLDTFLWS corresponding to C-Terminus according to NP_005870.2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRAIP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 4 - 6 ug/ml
  • Immunohistochemistry-Paraffin 4 - 6 ug/ml
  • Peptide ELISA Detection limit 1:1000
  • Western Blot
• Application Notes: Western Blot reactivity reported in scientific literature (PMID: 25335891).
• Control:

  TRAIP Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRAIP Antibody

• RNF206TRIPRING finger protein 206
• TRAF interacting protein
• TRAF-interacting protein

Background

The TNF receptor-associated factor (TRAF) family is a group of cytoplasmic adapter proteins that link a wide variety of cell surface receptors including the TNF and IL-1 receptor (TRNFR and IL-1R) superfamily to diverse signaling cascades involved in differentiation, proliferation, activation and apoptosis. TRIP (TRAF-interacting protein) was identified in 1997 as a component of receptor-TRAF signaling complexes (Lee, 1997). TRIP (also known as TRAIP) has been shown to associate with TRNFR2 or CD30 signaling complexes through its interaction with TRAF proteins and inhibit TRAF2-mediated NF-kB activation (Lee, 1997; Regamey et al, 2003). The recruitment of different TRAFs and TRAF associated proteins like TRIP to receptor complexes can help regulate and provide specificity to the intracellular signals triggered by the cell surface receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAIP Antibody (NB100-1230)(2)

Publications using NB100-1230

• Chapard C, Meraldi P, Gleich T et al. The TRAF-interacting protein (TRAIP) is a regulator of the spindle assembly checkpoint. J. Cell. Sci. 2014-10-21 [PMID: 25335891] (WB, Human)
  
  Details:
  TRAIP antibody used for analysis of TRAIP auto-ubiquitination with WB assay .
• Lee SY, Lee SY, Choi Y. TRAF-interacting protein (TRIP): a novel component of the tumor necrosis factor receptor (TNFR)- and CD30-TRAF signaling complexes that inhibits TRAF2-mediated NF-kappaB activation. J Exp Med 1997-04-07 [PMID: 9104814]

Bioinformatics

• Gene Symbol: TRAIP
• Entrez:
  • 10293(Human)
• Uniprot:
  • NP_005870.2(Human)