Human TRAIL/TNFSF10 ELISA Kit (Colorimetric)

ELISA: Human TRAIL/TNFSF10 ELISA Kit (Colorimetric) [NBP1-82418] - Representative standard curve for human TRAIL ELISA. Human TRAIL was diluted in serial 2-fold stepsin Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be run foreach group of microwell strips assayed.

Product Discontinued

Human TRAIL/TNFSF10 ELISA Kit (Colorimetric) Summary

• Description: This kit is an enzyme-linked immunosorbent assay for quantitative detection.
• Specificity: This antibody is reactive to the soluble protein. The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human TRAIL positive serum. There was no crossreactivity detected.
• Standard Curve Range: 15.6 - 1000 pg/mL
• Sensitivity: 5.0 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 7.7%
• Intra-Assay: CV% < 6.5%
• Spike Recovery: The amount of endogenous human TRAIL in unspiked serum was subtracted from the spike values. The overall mean recovery was 77%.
• Sample Volume: 50 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: TNFSF10

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human TRAIL/TNFSF10 ELISA Kit (Colorimetric)

• Apo-2 ligand
• APO2L
• Apo-2Ltumor necrosis factor (ligand) family, member 10
• APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
• CD253 antigen
• CD253
• Protein TRAIL
• TL2
• TNF-related apoptosis-inducing ligand
• TNFSF10
• TRAIL
• TRAILTNF-related apoptosis inducing ligand TRAIL
• tumor necrosis factor (ligand) superfamily, member 10
• tumor necrosis factor ligand superfamily member 10

Background

TRAIL (TNF-related-apoptosis-inducing-ligand) was found to be a member of the TNF family which mediates cell death in a wide variety of malignant cell lines and primary tumor cells. TRAIL induces two different signals, cell death mediated by caspases and gene induction mediated by NFkappaB. The cytotoxic ligand TRAIL interacts with five receptors, only two of which (TRAIL-R1 and R2) have a death domain. TRAIL, which is ubiquitous expressed, exhibits very complex signalling and tumor-selective induction of apoptosis. TRAIL is involved in lymphatic neoplastic disorders and thyroid diseases, and the treatment of cancer like melanoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for TRAIL/TNFSF10 ELISA Kit (NBP1-82418). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: TNFSF10