TRAIL/TNFSF10 Antibody (III6F) - BSA Free

Western Blot: TRAIL/TNFSF10 Antibody (III6F) [NBP1-97640] - Lane 1: Marker [kDa]. Lane 2: 100ng Recombinant Human TRAIL/TNFSF10 Protein (24kDa). Stained with mouse anti-human TRAIL (III6F; 1ug/mL) overnight at 4C, followed by IR dye 680 goat anti-mouse secondary (LI-COR#926-68070; 1:10,000) for 1 hour at room-temperature.

• Reactivity: Hu
• Applications: WB, ELISA, Flow, IHC, IP
• Clone: III6F
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

TRAIL/TNFSF10 Antibody (III6F) - BSA Free Summary

• Immunogen: Recombinant human soluble TRAIL.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for TRAIL/TNFSF10 Antibody (III6F) - BSA Free

• Apo-2 ligand
• APO2L
• Apo-2Ltumor necrosis factor (ligand) family, member 10
• APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
• CD253 antigen
• CD253
• Protein TRAIL
• TL2
• TNF-related apoptosis-inducing ligand
• TNFSF10
• TRAIL
• TRAILTNF-related apoptosis inducing ligand TRAIL
• tumor necrosis factor (ligand) superfamily, member 10
• tumor necrosis factor ligand superfamily member 10

Background

Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain". In addition, two decoy receptors for TRAIL have also been identified. These receptors, designated DcR1 and DcR2, lack the death domain. Binding of TRAIL to either of these receptors, therefore, does not transmit the death signal. Thus, these receptors represent a novel way of regulating cell sensitivity to a pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAIL/TNFSF10 Antibody (NBP1-97640)(2)

Publications using NBP1-97640

• Sanlioglu AD, Dirice E, Elpek O et al. High levels of endogenous tumor necrosis factor-related apoptosis-inducing ligand expression correlate with increased cell death in human pancreas. Pancreas. 2008-05-01 [PMID: 18437085]
• Liabakk NB, Sundan A, Torp S et al. Development, characterization and use of monoclonal antibodies against sTRAIL: measurement of sTRAIL by ELISA. J Immunol Methods. 2002-01-01 [PMID: 11730847]

Review for TRAIL/TNFSF10 Antibody (NBP1-97640) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 06/01/2018

Summary

• Application: Western Blot
• Sample Tested: Recombinant protein
• Species: Human
• Lot: A0055-109

Comments

• Comments: Stained with mouse anti-human TRAIL (III6F; 1ug/mL) overnight at 4C, followed by IRdye 680 goat anti-mouse secondary (LI-COR#926-68070; 1:10,000) for 1 hour at room-temperature.

Bioinformatics

• Gene Symbol: TNFSF10