TRAIL/TNFSF10 Antibody (75411) [Unconjugated]

In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (Catalog # MAB050) and the metabolic inhibitor actinomycin D (1 µg/mL), Recombinant Human TRAIL/TNFSF10 (Catalog # 375-TL) induces cytotoxicity in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Under these conditions, cytotoxicity elicited by Recombinant Human TRAIL/TNFSF10 (12 ng/mL) is neutralized (green line) by increasing concentrations of Human TRAIL/TNFSF10 Monoclonal Antibody (Catalog # MAB375). The ND50 is typically 2-8 ng/mL.

• Reactivity: Hu
• Applications: IHC, B/N
• Clone: 75411
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TRAIL/TNFSF10 Antibody (75411) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TRAIL/TNFSF10
  Thr95-Gly281
  Accession # P50591
• Specificity: Detects human TRAIL/TNFSF10 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF10
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Neutralization 2-8 ng/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAIL/TNFSF10 Antibody (75411) [Unconjugated]

• Apo-2 ligand
• APO2L
• Apo-2Ltumor necrosis factor (ligand) family, member 10
• APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
• CD253 antigen
• CD253
• Protein TRAIL
• TL2
• TNF-related apoptosis-inducing ligand
• TNFSF10
• TRAIL
• TRAILTNF-related apoptosis inducing ligand TRAIL
• tumor necrosis factor (ligand) superfamily, member 10
• tumor necrosis factor ligand superfamily member 10

Background

TRAIL is a type II transmembrane protein belonging to the TNF superfamily and is now designated TNFSF10. TRAIL is active as a homotrimer and is produced by a variety of cell types in both the membrane bound form and also as a soluble molecule. It binds to any of the four TRAIL receptors as well as to Osteoprotegerin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAIL/TNFSF10 Antibody (MAB375)(22)

Publications using MAB375

• Sánchez-Cerrillo, I;Agudo-Lera, M;Popova, O;Tsukalov, I;Calvet-Mirabent, M;de Los Santos, I;García-Fraile, L;Fuentes, P;Delgado-Arévalo, C;Alcain, J;Sánchez-Gaona, N;Grau-Expósito, J;Lázaro-Díez, M;Muñoz-Calleja, C;Alfranca, A;Genescà, M;Prado, JG;Vrbanac, V;Balazs, A;Buzón, MJ;Toribio, ML;Muñoz-Fernández, MA;Sánchez-Madrid, F;Martín-Gayo, E; Combined dendritic cell and anti-TIGIT immunotherapy potentiates adaptive NK cells against HIV-1 EMBO molecular medicine 2025-06-05 [PMID: 40473838] (Flow Cytometry, Human)
• AV Yagolovich, AA Isakova, AA Artykov, YV Vorontsova, DV Mazur, NV Antipova, MS Pavlyukov, MI Shakhparon, AM Gileva, EA Markvichev, EA Plotnikova, AA Pankratov, MP Kirpichnik, ME Gasparian, DA Dolgikh DR5-Selective TRAIL Variant DR5-B Functionalized with Tumor-Penetrating iRGD Peptide for Enhanced Antitumor Activity against Glioblastoma International Journal of Molecular Sciences, 2022-10-21;23(20):. 2022-10-21 [PMID: 36293545] (ELISA Detection, Human)
• AV Yagolovich, AA Artykov, AA Isakova, YV Vorontsova, DA Dolgikh, MP Kirpichnik, ME Gasparian Optimized Heterologous Expression and Efficient Purification of a New TRAIL-Based Antitumor Fusion Protein SRH-DR5-B with Dual VEGFR2 and DR5 Receptor Specificity International Journal of Molecular Sciences, 2022-05-24;23(11):. 2022-05-24 [PMID: 35682540] (ELISA Capture, Human)
• N Rajaratnam, NE Ditlevsen, JK Sloth, R Bæk, MM Jørgensen, OB Christians Extracellular Vesicles: An Important Biomarker in Recurrent Pregnancy Loss? Journal of Clinical Medicine, 2021-06-09;10(12):. 2021-06-09 [PMID: 34207656] (IHC, Human)
• G Wu, C Cheney, Q Huang, DJ Hazuda, BJ Howell, P Zuck Improved Detection of HIV Gag p24 Protein Using a Combined Immunoprecipitation and Digital ELISA Method Frontiers in Microbiology, 2021-03-16;12(0):636703. 2021-03-16 [PMID: 33796087] (ELISA (Capture), Human)
• N Lalaoui, D Merino, G Giner, F Vaillant, D Chau, L Liu, T Kratina, B Pal, JR Whittle, N Etemadi, J Berthelet, J Gräsel, C Hall, ME Ritchie, M Ernst, GK Smyth, DL Vaux, JE Visvader, GJ Lindeman, J Silke Targeting triple-negative breast cancers with the Smac-mimetic birinapant Cell Death Differ., 2020-04-27;0(0):. 2020-04-27 [PMID: 32341449] (Neutralization, Human)
• MM Jørgensen, R Bæk, J Sloth, K Varming, OB Christians, NE Ditlevsen, N Rajaratnam Treatment with intravenous immunoglobulin increases the level of small EVs in plasma of pregnant women with recurrent pregnancy loss J. Reprod. Immunol., 2020-04-09;140(0):103128. 2020-04-09 [PMID: 32330775] (Bioassay, Human)
• ND Arnold, JA Pickworth, LE West, S Dawson, JA Carvalho, H Casbolt, AT Braithwait, J Iremonger, L Renshall, V Germaschew, M McCourt, P Bland-Ward, H Kowash, AG Hameed, AMK Rothman, MG Frid, AA Roger Thom, HR Evans, M Southwood, NW Morrell, DC Crossman, MKB Whyte, KR Stenmark, CM Newman, DG Kiely, SE Francis, A Lawrie A therapeutic antibody targeting osteoprotegerin attenuates severe experimental pulmonary arterial hypertension Nat Commun, 2019-11-15;10(1):5183. 2019-11-15 [PMID: 31729368] (Neutralization, Mouse)
• RE Vickman, J Yang, NA Lanman, GM Cresswell, F Zheng, C Zhang, RW Doerge, SA Crist, AD Mesecar, CD Hu, TL Ratliff Cholesterol Sulfotransferase SULT2B1b Modulates Sensitivity to Death Receptor Ligand TNF alpha in Castration Resistant Prostate Cancer Mol. Cancer Res., 2019-03-01;0(0):. 2019-03-01 [PMID: 30824526] (Neutralization, Human)
• G Brunetti, A Di Benedet, F Posa, G Colaianni, MF Faienza, A Ballini, S Colucci, G Passeri, L Lo Muzio, M Grano, G Mori High expression of TRAIL by osteoblastic differentiated dental pulp stem cells affects myeloma cell viability Oncol. Rep., 2018-02-15;0(0):. 2/15/2018 [PMID: 29484421]
• MC Tanzer, N Khan, JA Rickard, N Etemadi, N Lalaoui, SK Spall, JM Hildebrand, D Segal, M Miasari, D Chau, WL Wong, M McKinlay, SK Chunduru, CA Benetatos, SM Condon, JE Vince, MJ Herold, J Silke Combination of IAP antagonist and IFN? activates novel caspase-10- and RIPK1-dependent cell death pathways Cell Death Differ, 2017-01-20;0(0):. 2017-01-20 [PMID: 28106882] (Neutralization, Human)
• Negative control of TRAIL-R1 signaling by transforming growth factor ?1 in pancreatic tumor cells involves Smad-dependent down regulation of TRAIL-R1 Cell Signal, 2016-08-01;28(11):1652-1662. 2016-08-01 [PMID: 27492861] (Neutralization, Human)
• S V Singh, A K Ajay, N Mohammad, P Malvi, B Chaube, A S Meena et al. Proteasomal inhibition sensitizes cervical cancer cells to mitomycin C-induced bystander effect: the role of tumor microenvironment Cell Death & Disease 2015-10-22 [PMID: 26492368]
• Robinet P, Baychelier F, Fontaine T, Picard C, Debre P, Vieillard V, Latge J, Elbim C A polysaccharide virulence factor of a human fungal pathogen induces neutrophil apoptosis via NK cells. J Immunol, 2014-04-30;192(11):5332-42. 2014-04-30 [PMID: 24790151] (Neutralization, Human)
• Tel J, Smits E, Anguille S, Joshi R, Figdor C, de Vries I Human plasmacytoid dendritic cells are equipped with antigen-presenting and tumoricidal capacities. Blood, 2012-09-10;120(19):3936-44. 2012-09-10 [PMID: 22966165] (Bioassay, Human)
• Kalb ML, Glaser A, Stary G, Koszik F, Stingl G TRAIL(+) human plasmacytoid dendritic cells kill tumor cells in vitro: mechanisms of imiquimod- and IFN-alpha-mediated antitumor reactivity. J. Immunol., 2012-01-09;188(4):1583-91. 2012-01-09 [PMID: 22231699] (Neutralization, Human)
• Inglefield JR, Dumitru CD, Alkan SS, Gibson SJ, Lipson KE, Tomai MA, Larson CJ, Vasilakos JP TLR7 Agonist 852A Inhibition of Tumor Cell Proliferation Is Dependent on Plasmacytoid Dendritic Cells and Type I IFN. J. Interferon Cytokine Res., 2008-04-01;28(4):253-63. 2008-04-01 [PMID: 18439103] (Neutralization, Human)
• Kavurma MM, Schoppet M, Bobryshev YV, Khachigian LM, Bennett MR TRAIL stimulates proliferation of vascular smooth muscle cells via activation of NF-kappaB and induction of insulin-like growth factor-1 receptor. J. Biol. Chem., 2008-01-03;283(12):7754-62. 2008-01-03 [PMID: 18178561] (IHC-P, Human)
• Krysov SV, Krysov</LastName><ForeNam SV</Initia, Rowley TF, Al-Shamkhani A Inhibition of p38 mitogen-activated protein kinase unmasks a CD30-triggered apoptotic pathway in anaplastic large cell lymphoma cells. Mol. Cancer Ther., 2007-02-01;6(2):703-11. 2007-02-01 [PMID: 17308066] (Neutralization, Human)
• Georgopoulos NT, Steele LP, Thomson MJ, Selby PJ, Southgate J, Trejdosiewicz LK A novel mechanism of CD40-induced apoptosis of carcinoma cells involving TRAF3 and JNK/AP-1 activation. Cell Death Differ., 2006-01-20;13(10):1789-801. 2006-01-20 [PMID: 16429118] (Neutralization, Human)
• Brando C, Mukhopadhyay S, Kovacs E, Medina R, Patel P, Catina TL, Campbell KS, Santoli D Receptors and lytic mediators regulating anti-tumor activity by the leukemic killer T cell line TALL-104. J. Leukoc. Biol., 2005-06-03;78(2):359-71. 2005-06-03 [PMID: 15937142] (Neutralization, Human)
• Armeanu S, Lauer UM, Smirnow I, Schenk M, Weiss TS, Gregor M, Bitzer M Adenoviral gene transfer of tumor necrosis factor-related apoptosis-inducing ligand overcomes an impaired response of hepatoma cells but causes severe apoptosis in primary human hepatocytes. Cancer Res., 2003-05-15;63(10):2369-72. 2003-05-15 [PMID: 12750253] (ICC, Human)

Bioinformatics

• Gene Symbol: TNFSF10
• Uniprot:
  • P50591()