TRAIL/TNFSF10 Antibody (75411.11)

Product Discontinued

TRAIL/TNFSF10 Antibody (75411.11) Summary

• Immunogen: Recombinant full length protein (Human).
• Localization: Cell Membrane
• Specificity: In immunoblots, this demonstrates approximately 5% cross-reactivity with recombinant human Trance and recombinant human TNF alpha.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFSF10
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.5-1 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA and western blot. ELISA dilution determined using 25ng/well recombinant human TRAIL. Western blot detects a band of approximately 24 kDa (predicted molecular weight: 33.7 kDa). Determined using human TRAIL at approximately 50 ng/lane under non-reducing and reducing conditions. Inhibition: Monoclonal anti-TRAIL neutralizes bioactivity on mouse L929 cells. To measure the ability of this antibody to neutralize the biological activity of TRAIL, cross-linked recombinant human TRAIL is incubated with various concentrations of the antibody for 1 hour at 37C.
• Control:

  TRAIL/TNFSF10 Overexpression Lysate

Reactivity Notes

Cross-reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10 mM Sodium Phosphate (pH 7.6), 0.25M NaCl and 15 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Alternate Names for TRAIL/TNFSF10 Antibody (75411.11)

• Apo-2 ligand
• APO2L
• Apo-2Ltumor necrosis factor (ligand) family, member 10
• APO2Ltumor necrosis factor apoptosis-inducing ligand splice variant delta
• CD253 antigen
• CD253
• Protein TRAIL
• TL2
• TNF-related apoptosis-inducing ligand
• TNFSF10
• TRAIL
• TRAILTNF-related apoptosis inducing ligand TRAIL
• tumor necrosis factor (ligand) superfamily, member 10
• tumor necrosis factor ligand superfamily member 10

Background

Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain". In addition, two decoy receptors for TRAIL have also been identified. These receptors, designated DcR1 and DcR2, lack the death domain. Binding of TRAIL to either of these receptors, therefore, does not transmit the death signal. Thus, these receptors represent a novel way of regulating cell sensitivity to a pro-apoptotic cytokine at the cell surface. TRAIL is expressed predominantly in spleen, lung, and prostate but also in many other tissues. TRAIL is a type II membrane protein from the TNF family. The human form of the protein is 281 amino acids in length, whereas the murine form is 291 amino acids. The two forms share 65% identity. Recombinant human TRAIL monomer has a molecular mass of approximately 21 kDa. Due to glycosylation, recombinant human TRAIL migrates as a 24 kDa protein in SDS PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFSF10
• Entrez:
  • 8743(Human)