TRAFD1 Antibody (8E6E7) - BSA Free

Western Blot: TRAFD1 Antibody (8E6E7) [NBP2-37408] - Western blot analysis using TRAFD1 mouse mAb against HEK293 (1), Raji (2), and Jurkat (3) cell lysate.

Immunocytochemistry/Immunofluorescence: TRAFD1 Antibody (8E6E7) [NBP2-37408] - Immunofluorescence analysis of HepG2 cells using TRAFD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.

Flow Cytometry: TRAFD1 Antibody (8E6E7) [NBP2-37408] - Flow cytometric analysis of HeLa cells using TRAFD1 mouse mAb (green) and negative control (purple).

Western Blot: TRAFD1 Antibody (8E6E7) [NBP2-37408] - Western blot analysis using TRAFD1 mAb against human TRAFD1 recombinant protein. (Expected MW is 45 kDa)

Western Blot: TRAFD1 Antibody (8E6E7) [NBP2-37408] - Western blot analysis using TRAFD1 mAb against HEK293 (1) and TRAFD1 (AA: 401-582)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: TRAFD1 Antibody (8E6E7) [NBP2-37408] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, ICC/IF, CyTOF-ready
• Clone: 8E6E7
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TRAFD1 Antibody (8E6E7) - BSA Free Summary

• Description: Novus Biologicals Mouse TRAFD1 Antibody (8E6E7) - BSA Free (NBP2-37408) is a monoclonal antibody validated for use in WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human TRAFD1 (AA: 401-582) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRAFD1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 64.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for TRAFD1 Antibody (8E6E7) - BSA Free

• FLN29FLN29 gene product
• Protein FLN29
• TRAF-type zinc finger domain containing 1
• TRAF-type zinc finger domain-containing protein 1

Background

The innate immune system confers host defense against viral and microbial infection, and TRAFD1 is a negative feedback regulator that controls excessive immune responses (Sanada et al., 2008 (PubMed 18849341)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAFD1