TRAF-3 Antibody (433113)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A20 mouse B cell lymphoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse/Rat TRAF-3 Isoform 2 Monoclonal Antibody (Catalog # MAB3278) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for TRAF-3 Isoform 2 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 433113
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

TRAF-3 Antibody (433113) Summary

• Immunogen: E. coli-derived recombinant human TRAF-3
  Met1-Pro543
  Accession # NP_663778
• Specificity: Detects endogenous human, mouse, and rat TRAF-3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human TRAF-1, -2, -4, -5, or -6.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TRAF3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAF-3 Antibody (433113)

• CAP1
• CAP-1CD40 receptor associated factor 1
• CD40 associated protein 1
• CD40 binding protein
• CD40 receptor-associated factor 1
• CD40-binding protein
• CD40bp
• CRAF1
• CRAF1CAP1
• LAP1
• LAP1CD40BP
• LMP1-associated protein 1
• TNF receptor-associated factor 3
• TRAF3
• TRAF-3

Background

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Seven members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2‑6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. Two isoforms of TRAF-3 have been identified, a 568 amino acid, 64 kDa protein (isoform 1, also known as CD40BP and CRAF1) and a 543 amino acid, 62 kDa protein (isoform 2, also known as CAP1 and LAP1). TRAF-3 has been shown bind specifically to the PEQET sequence motif on the cytoplasmic domain of CD40. TRAF-3 and TRAF-6 have also been identified as being involved in TLR signaling events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAF3
• Uniprot:
  • NP_663778()