TRAF-3 Antibody (433113) [CoraFluor™ 1]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 433113
• Clonality: Monoclonal
• Host: Rat
• Conjugate: CoraFluor 1

TRAF-3 Antibody (433113) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: E. coli-derived recombinant human TRAF-3
  Met1-Pro543
  Accession # NP_663778
• Specificity: Detects endogenous human, mouse, and rat TRAF-3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human TRAF-1, -2, -4, -5, or -6.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: TRAF3
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for TRAF-3 Antibody (433113) [CoraFluor™ 1]

• CAP1
• CAP-1CD40 receptor associated factor 1
• CD40 associated protein 1
• CD40 binding protein
• CD40 receptor-associated factor 1
• CD40-binding protein
• CD40bp
• CRAF1
• CRAF1CAP1
• LAP1
• LAP1CD40BP
• LMP1-associated protein 1
• TNF receptor-associated factor 3
• TRAF3
• TRAF-3

Background

Human TRAF-3 is a signaling molecule that interacts with the cytoplasmic tails of CD40 and other TNF-receptor family members (1). The TNF-receptor-associated factor (TRAF) family is a phylogenetically conserved group of scaffold proteins that link receptors of the IL-1R/Toll and TNF receptor family to signalling cascades, leading to the activation of NF-kappaB and mitogen-activated protein kinases. Furthermore, TRAF proteins serve as a docking platform for a variety of regulators of these signaling pathways and are themselves often regulated at the transcriptional and posttranslational level (2). The CD40 fragment binds as a hairpin loop across the surface of the TRAF domain. Residues shown by mutagenesis and deletion analysis to be critical for TRAF3 binding are involved either in direct contact with TRAF3 or in intramolecular interactions that stabilize the hairpin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAF3