TRAF-1 Antibody

Western Blot: TRAF-1 Antibody [NB100-2822] - Analysis of TRAF-1 using NB100-2822 at 0.5ug/ml in U937 cell lysate (35ug protein in RIPA buffer).

Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-2822] - staining of paraffin embedded Human Thymus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: TRAF-1 Antibody [NB100-2822] - staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRAF-1 Antibody Summary

• Description: Novus Biologicals Goat TRAF-1 Antibody (NB100-2822) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-TRAF-1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-KLQSPKHAYVKDD corresponding to C-Terminus according to NP_005649.1.
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 10 ug/ml
• Application Notes: WB: Approx. 50-55 kDa band observed in lysates of monocytic leukemia cell line U937 (PMID: 10657935).
  IHC: Paraffin embedded Human Thymus and Small Intestine.
• Control:

  TRAF-1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRAF-1 Antibody

• EBI6
• EBI6MGC:10353
• Epstein-Bar virus-induced protein 6
• Epstein-Barr virus-induced protein 6
• TNF receptor-associated factor 1
• TRAF1
• TRAF-1

Background

The protein encoded by this gene is a member of the TNF receptor (TNFR) associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from various receptors of the TNFR superfamily. This protein and TRAF2 form a heterodimeric complex, which is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF2 also interacts with inhibitor-of-apoptosis proteins (IAPs), and thus mediates the anti-apoptotic signals from TNF receptors. The expression of this protein can be induced by Epstein-Barr virus (EBV). EBV infection membrane protein 1 (LMP1) is found to interact with this and other TRAF proteins; this interaction is thought to link LMP1-mediated B lymphocyte transformation to the signal transduction from TNFR family receptors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAF-1 Antibody (NB100-2822)(2)

Publications using NB100-2822

• Su X, Li S, Meng M et al. TNF receptor-associated factor-1 (TRAF1) negatively regulates Toll/IL-1 receptor domain-containing adaptor inducing IFN-beta (TRIF)-mediated signaling. Eur J Immunol 2006-01-01 [PMID: 16323247]
• Pryhuber GS, Huyck HL, Staversky RJ et al. Tumor necrosis factor-alpha-induced lung cell expression of antiapoptotic genes TRAF1 and cIAP2. Am J Respir Cell Mol Biol 2000-02-01 [PMID: 10657935]

Bioinformatics

• Gene Symbol: TRAF1
• Entrez:
  • 7185(Human)
  • 22029(Mouse)
• Uniprot:
  • NP_005649.1(Human)