TRAF-1 Antibody

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human TRAF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3276) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional refer-ence, recombinant human TRAF-1, TRAF-2, TRAF-3, TRAF-4, TRAF-5, and TRAF-6 (2 ng/lane) were included. A specific band for TRAF-1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TRAF-1 Antibody Summary

• Immunogen: E. coli-derived recombinant human TRAF-1
  Met1-Thr416
  Accession # Q13077
• Specificity: Detects human TRAF-1 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human TRAF-2, -3, -4, -5, and -6 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRAF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAF-1 Antibody

• EBI6
• EBI6MGC:10353
• Epstein-Bar virus-induced protein 6
• Epstein-Barr virus-induced protein 6
• TNF receptor-associated factor 1
• TRAF1
• TRAF-1

Background

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Six members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAF-1, also known as EBI6, is a 416 amino acid, 46 kDa protein that is a unique member of the TRAF family, in that it lacks the N-terminal RING finger domain common in TRAFs 2-6. TRAF-1 interacts with the cytoplasmic domain of TNFR2 and other TNFR family members to mediate downstream signaling events. TRAF-1 is a substrate for caspases activated by TNF family death receptors. TRAF-1 can homodimerize as well as form heterodimers with TRAF-2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRAF1
• Uniprot:
  • Q13077()