TPX2 Antibody (TPX2-01) - BSA Free

Western Blot: TPX2 Antibody (TPX2-01) [NBP2-67265] - Analysis of TPX2 using monoclonal antibody TPX2 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.

Immunocytochemistry: TPX2 Antibody (TPX2-01) [NBP2-67265] - Glioblastoma cell line T98G: a) interphase, b) mitosis. Bar: 20 um.

Immunohistochemistry: TPX2 Antibody (TPX2-01) [NBP2-67265] - TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 um.

Immunoprecipitation: TPX2 Antibody (TPX2-01) [NBP2-67265] - TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2. 1) nuclear extract, 2) antibody, 3) immunoprecipitate, 4) carrier

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IP
• Clone: TPX2-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

TPX2 Antibody (TPX2-01) - BSA Free Summary

• Immunogen: Human TPX2
• Specificity: The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPX2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TPX2 Antibody (TPX2-01) - BSA Free

• C20orf1HCTP4
• C20orf2
• chromosome 20 open reading frame 1
• Differentially expressed in cancerous and non-cancerous lung cells 2
• differentially expressed in lung cells
• DIL2
• DIL-2FLS353
• HCA519
• Hepatocellular carcinoma-associated antigen 519
• P100
• p100GD:C20orf1
• preferentially expressed in colorectal cancer
• Protein fls353
• REPP86
• restricted expression proliferation associated protein 100
• Restricted expression proliferation-associated protein 100
• targeting protein for Xklp2
• TPX2, microtubule-associated protein homolog
• TPX2, microtubule-associated, homolog (Xenopus laevis)

Background

TPX2 is a microtubule cross-linking protein involved in spindle formation. During mitosis, it is strictly associated with the mitotic spindle, whereas during phases S and G2 it is diffusely distributed throughout the nucleus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TPX2 Antibody (NBP2-67265)(1)

Publication using NBP2-67265

• Zhang Y, Fan B, Li X et al. Phosphorylation of adducin-1 by TPX2 promotes interpolar microtubule homeostasis and precise chromosome segregation in mouse oocytes Cell & bioscience 2022-12-20 [PMID: 36539904] (ICC/IF, WB, Mouse)

Bioinformatics

• Gene Symbol: TPX2