TOX3 Antibody (TOX3/1123)

Western Blot: TOX3 Antibody (TOX3/1123) [NBP2-45165] - Western Blot Analysis of A431 cell lysate using TOX3 Mouse Monoclonal Antibody (TOX3/1123).

Immunofluorescence: TOX3 Antibody (TOX3/1123) [NBP2-45165] - E4.5 embryonic chicken neural tube. TOX3 antibody at 1:200 dilution. IF image submitted by a verified customer review.

Protein Array: TOX3 Antibody (TOX3/1123) [NBP2-45165] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TOX3 Mouse Monoclonal Antibody (TOX3/1123).

• Reactivity: Hu, Ch
• Applications: WB, ELISA, IHC, IHC-P, PA, ICC/IF
• Clone: TOX3/1123
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TOX3 Antibody (TOX3/1123) Summary

• Immunogen: Recombinant fragment of human TOX3 protein (around aa 251-389) (Exact sequence is proprietary) (Uniprot: O15405)
• Localization: Cytoplasmic & Nuclear
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOX3
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunofluorescence 0.5 - 1.0 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: ELISA: For coating, order Ab without BSA.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 63 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken reactivity reported from a verified customer review.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 1 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47748)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for TOX3 Antibody (TOX3/1123)

• CAG trinucleotide repeat-containing gene F9 protein
• CAGF9trinucleotide repeat containing 9
• TNRC9
• TOX high mobility group box family member 3
• Trinucleotide repeat-containing gene 9 protein

Background

The protein encoded by the TOX3 gene contains an HMG-box, indicating that it may be involved in bending and unwinding of DNA and alteration of chromatin structure. The C-terminus of the encoded protein is glutamine-rich due to CAG repeats in the coding sequence. A minor allele of this gene has been implicated in an elevated risk of breast cancer. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOX3 Antibody (NBP2-45165)(1)

Publication using NBP2-45165

• Estermann MA, Major AT, Smith CA DMRT1 regulation of TOX3 modulates expansion of the gonadal steroidogenic cell lineage in the chicken embryo Development (Cambridge, England) 2023-02-16 [PMID: 36794750] (IHC-Fr)

Review for TOX3 Antibody (NBP2-45165) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Avian and Chicken.

reviewed by: Martin Estermann Immunofluorescence-Frozen Avian and Chicken 10/07/2019

Summary

• Application: Other
• Sample Tested: E4.5 chicken embryo,Embryonic neural tube
• Species: Avian and Chicken
• Lot: 27324-1P180913

Comments

• Comments: Citrate Antigen Retrieval – 98°C; 30 minutes(low pH)

Bioinformatics

• Gene Symbol: TOX3
• Uniprot:
  • O15405(Human)