TORC3/CRTC3 Antibody (5G9)

Western Blot: TORC3/CRTC3 Antibody (5G9) [NBP2-37635] - Western blot analysis using CRTC3 mouse mAb against Hela (1), Jurkat (2), Cos7 (3) and MCF-7 (4) cell lysate.

Immunocytochemistry/Immunofluorescence: TORC3/CRTC3 Antibody (5G9) [NBP2-37635] - Immunofluorescence analysis of NTERA-2 cells using CRTC3 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry: TORC3/CRTC3 Antibody (5G9) [NBP2-37635] - Immunohistochemical analysis of paraffin-embedded lung cancer (left) and esophagus cancer (right) using CRTC3 mouse mAb with DAB staining.

Immunohistochemistry: TORC3/CRTC3 Antibody (5G9) [NBP2-37635] - Immunohistochemical analysis of paraffin-embedded breast cancer (left) and ovarian cancer (right) using CRTC3 mouse mAb with DAB staining.

ELISA: TORC3/CRTC3 Antibody (5G9) [NBP2-37635] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

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TORC3/CRTC3 Antibody (5G9) Summary

• Immunogen: Purified recombinant fragment of human CRTC3 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRTC3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for TORC3/CRTC3 Antibody (5G9)

• CREB regulated transcription coactivator 3
• CRTC3
• FLJ21868
• TORC3
• TORC-3
• TORC3CREB-regulated transcription coactivator 3
• Transducer of CREB protein 3
• transducer of regulated cAMP response element-binding protein (CREB) 3
• Transducer of regulated cAMP response element-binding protein 3
• transducer of regulated CREB protein 3

Background

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMPresponse element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active whendephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 withTAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potentcoactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activationof the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for TORC3/CRTC3 Antibody (NBP2-37635). (Showing 1 - 1 of 1 FAQ).

1. Can you please tell me where the epitope of this antibody lies inside CRTC3?
   • we only have the range of the immunogen as we have not mapped the epitope. The range is large aa 37-325 

Bioinformatics

• Gene Symbol: CRTC3