TOPORS Antibody

Western Blot: TOPORS Antibody [NBP1-22976] - Whole cell lysate from HeLa cells. Affinity purified rabbit anti-TOPORS antibody NBP1-22976. TOPORS was also immunoprecipitated by another rabbit anti-TOPORS antibody.

Immunohistochemistry-Paraffin: TOPORS Antibody [NBP1-22976] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- TOPORS used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

TOPORS Antibody Summary

• Description: Novus Biologicals Rabbit TOPORS Antibody (NBP1-22976) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-TOPORS Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 995 and 1045 of human topoisomerase I binding, arginine/serine-rich using the numbering given in entry topoisomerase I binding, arginine/serine-rich NP_005793.2 (GeneID 10210).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TOPORS
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TOPORS Antibody

• EC 6.3.2.-
• LUNtopoisomerase I binding, arginine/serine-rich
• p53-binding protein 3
• p53BP3
• retinitis pigmentosa 31 (autosomal dominant)
• RP31
• SUMO1-protein E3 ligase Topors
• topoisomerase I binding, arginine/serine-rich, E3 ubiquitin protein ligase
• Topoisomerase I-binding arginine/serine-rich protein
• Topoisomerase I-binding RING finger protein
• TP53BPLE3 ubiquitin-protein ligase Topors
• Tumor suppressor p53-binding protein 3

Background

TOPORS (topoisomerase I-binding RS protein) was identified in a a yeast two-hybrid screen for proteins that bind the N-terminus of topoisomerase I. TOPORS contains a RING-type zinc-finger domain and an RS-rich region. TOPORS has been found to possess both ubiquitin and SUMO-1 E3 ligase activity. It can ubiquitinate and sumoylate p53 and may possibly function as a tumor suppressor. Defects in TOPORS are the cause of retinitis pigmentosa type 31.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOPORS Antibody (NBP1-22976)(1)

Publication using NBP1-22976

• Amhaz S, Boëda B, Chouchène M et al. The UAS thioredoxin-like domain of UBXN7 regulates E3 ubiquitin ligase activity of RNF111/Arkadia BMC biology 2023-04-07 [PMID: 37024974]

Bioinformatics

• Gene Symbol: TOPORS
• Entrez:
  • 10210(Human)
• Uniprot:
  • Q9NS56(Human)