FoxF1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxF1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human FoxF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4798) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FoxF1 at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated

FoxF1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human FoxF1
  Met154-Met379
  Accession # Q12946
• Specificity: Detects human FoxF1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: FOXF1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for FoxF1 Antibody [Unconjugated]

• FKHL5
• FKHL5MGC105125
• forkhead box F1
• forkhead box protein F1
• Forkhead, drosophila, homolog-like 5
• Forkhead-related activator 1
• Forkhead-related protein FKHL5
• Forkhead-related transcription factor 1
• FoxF1
• FREAC-1
• FREAC1ACDMPV

Background

FoxF1 belongs to a large family of proteins that share a common forkhead/winged helix DNA binding domain. FoxF1 is implicated in pulmonary morphogenesis, specifically in development of the lung mesenchyme. Experiments in mice indicate that haploinsufficiency of FoxF1 can lead to perinatal lethality due to pulmonary abnormalities. In addition expression of FoxF1 can be induced by hedgehog ligands and appears to regulate expression of BMP-4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for FoxF1 Antibody (AF4798)(10)

Publications using AF4798

• Bian, F;Lan, YW;Zhao, S;Deng, Z;Shukla, S;Acharya, A;Donovan, J;Le, T;Milewski, D;Bacchetta, M;Hozain, AE;Tipograf, Y;Chen, YW;Xu, Y;Shi, D;Kalinichenko, VV;Kalin, TV; Lung endothelial cells regulate pulmonary fibrosis through FOXF1/R-Ras signaling Nature communications 2023-05-04 [PMID: 37137915] (Western Blot, Human)
• AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells Cell Stem Cell, 2021-12-01;0(0):. 2021-12-01 [PMID: 34856121] (IHC, Human, Mouse)
• K Sasaki, A Oguchi, K Cheng, Y Murakawa, I Okamoto, H Ohta, Y Yabuta, C Iwatani, H Tsuchiya, T Yamamoto, Y Seita, M Saitou The embryonic ontogeny of the gonadal somatic cells in mice and monkeys Cell Reports, 2021-05-04;35(5):109075. 2021-05-04 [PMID: 33951437] (IHC, Mouse)
• D Milewski, S Shukla, BE Gryder, A Pradhan, J Donovan, P Sudha, S Vallabh, A Pyros, Y Xu, A Barski, S Szabo, B Turpin, JG Pressey, DP Millay, J Khan, VV Kalinichen, TV Kalin FOXF1 is required for the oncogenic properties of PAX3-FOXO1 in rhabdomyosarcoma Oncogene, 2021-02-24;0(0):. 2021-02-24 [PMID: 33627785] (Immunoprecipitation, Human)
• DG Belair, G Lu, LE Waller, JA Gustin, ND Collins, KL Kolaja Thalidomide Inhibits Human iPSC Mesendoderm Differentiation by Modulating CRBN-dependent Degradation of SALL4 Sci Rep, 2020-02-18;10(1):2864. 2020-02-18 [PMID: 32071327] (ICC, Human)
• J Xu, H Liu, Y Lan, M Adam, DE Clouthier, S Potter, R Jiang Hedgehog signaling patterns the oral-aboral axis of the mandibular arch Elife, 2019-01-14;8(0):. 2019-01-14 [PMID: 30638444] (IHC-P, Mouse)
• M Black, D Milewski, T Le, X Ren, Y Xu, VV Kalinichen, TV Kalin FOXF1 Inhibits Pulmonary Fibrosis by Preventing CDH2-CDH11 Cadherin Switch in Myofibroblasts Cell Rep, 2018-04-10;23(2):442-458. 2018-04-10 [PMID: 29642003] (ChIP, Mouse)
• KW McCracken, E Aihara, B Martin, CM Crawford, T Broda, J Treguier, X Zhang, JM Shannon, MH Montrose, JM Wells Wnt/?-catenin promotes gastric fundus specification in mice and humans Nature, 2017-01-04;0(0):. 2017-01-04 [PMID: 28052057] (IHC-P, Mouse)
• FoxF1 and FoxF2 transcription factors synergistically promote rhabdomyosarcoma carcinogenesis by repressing transcription of p21(Cip1) CDK inhibitor Oncogene, 2016-07-18;0(0):. 2016-07-18 [PMID: 27425595] (Western Blot, Mouse)
• Rankin S, Thi Tran H, Wlizla M, Mancini P, Shifley E, Bloor S, Han L, Vleminckx K, Wert S, Zorn A A Molecular atlas of Xenopus respiratory system development. Dev Dyn, 2014-09-11;244(1):69-85. 2014-09-11 [PMID: 25156440] (IHC-P, Xenopus)

Bioinformatics

• Gene Symbol: FOXF1
• Uniprot:
  • Q12946()