Topoisomerase I Antibody

Western Blot: Topoisomerase I Antibody [NBP1-30481] - Whole cell lysate from HeLa and 293T cells. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482.

• Reactivity: Hu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Topoisomerase I Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 100 and 150 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TOP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Topoisomerase I Antibody

• DNA topoisomerase 1
• DNA topoisomerase I
• EC 5.6.2.1
• EC 5.99.1.2
• TOP1
• TOPI
• topoisomerase (DNA) I
• Topoisomerase I
• type I DNA topoisomerase

Background

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Topoisomerase I Antibody (NBP1-30481)(2)

Publications using NBP1-30481

• Kim PY, Tan O, Liu B et al. High TDP43 expression is required for TRIM16-induced inhibition of cancer cell growth and correlated with good prognosis of neuroblastoma and breast cancer patients. Cancer Lett. 2016-02-20 [PMID: 26902425] (WB, Human)
• Kim PY, Rahmanto AS, Tan O et al. TRIM16 overexpression induces apoptosis through activation of caspase-2 in cancer cells. Apoptosis 2013-02-13 [PMID: 23404198] (WB, Human)

FAQs for Topoisomerase I Antibody (NBP1-30481). (Showing 1 - 1 of 1 FAQs).

1. Do you have any data about the reactivity of this antibody against mouse Top1. I want to use this antibody for both mouse and human Top1. Your description says it is reactive against human Top1. Will it also equally work against Mouse Top1?
   • Since the immunogen of NBP1-30481 maps to a region between residues 100-150 of human DNA topoisomerase I, based on the sequence identity, I would not recommend it for mouse samples. I did a blast search on the immunogen and unfortunately there is not much homology with mouse Top1.

Bioinformatics

• Gene Symbol: TOP1
• Entrez:
  • 7150(Human)
• Uniprot:
  • P11387(Human)