TOP2A Antibody

Immunohistochemistry: TOP2A Antibody [NB110-40636] - Sample: FFPE section of human larynx squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Topo II Alpha used at a dilution of 1:250. Detection: DAB staining using anti-Rabbit IHC antibody at a dilution of 1:100.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.25 mg/ml

TOP2A Antibody Summary

• Description: Novus Biologicals Rabbit TOP2A Antibody (NB110-40636) is a polyclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1500 and the C-terminus (residue 1531) of human Topoisomerase (DNA) II Alpha using the numbering given in entry NP_001058.2 (GeneID 7153).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TOP2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.25 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TOP2A Antibody

• DNA topoisomerase (ATP-hydrolyzing)
• DNA topoisomerase 2-alpha
• DNA topoisomerase II, 170 kD
• DNA topoisomerase II, alpha isozyme
• EC 5.99.1.3
• TOP2
• TOP2A
• TOP2DNA gyrase
• topoisomerase (DNA) II alpha (170kD)
• topoisomerase (DNA) II alpha 170kDa
• TP2A

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOP2A
• Entrez:
  • 7153(Human)
• Uniprot:
  • NP_001058.2(Human)