TOP2A Antibody (TOP2A/4397R)

Images

 
Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/4397R) [NBP3-07735] - Formalin-fixed, paraffin-embedded human tonsil stained with TOP2A Recombinant Rabbit Monoclonal Antibody (TOP2A/4397R).
Immunohistochemistry-Paraffin: TOP2A Antibody (TOP2A/4397R) [NBP3-07735] - Formalin-fixed, paraffin-embedded human lymph node stained with TOP2A Recombinant Rabbit Monoclonal Antibody (TOP2A/4397R).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P
Clone
TOP2A/4397R
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

TOP2A Antibody (TOP2A/4397R) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
Synthetic peptide corresponding to TOP2A residues within aa1431-1531 of TOP2A (Uniprot: P11388 )
Localization
Nuclear
Marker
Proliferation & Drug-Resistance Marker
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
TOP2A
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08756)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.

Alternate Names for TOP2A Antibody (TOP2A/4397R)

  • DNA topoisomerase (ATP-hydrolyzing)
  • DNA topoisomerase 2-alpha
  • DNA topoisomerase II, 170 kD
  • DNA topoisomerase II, alpha isozyme
  • EC 5.99.1.3
  • TOP2
  • TOP2A
  • TOP2DNA gyrase
  • topoisomerase (DNA) II alpha (170kD)
  • topoisomerase (DNA) II alpha 170kDa
  • TP2A

Background

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol TOP2A
Uniprot