TOP2A Antibody (TOP2A/4397R) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Applications: IHC
• Clone: TOP2A/4397R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 555

TOP2A Antibody (TOP2A/4397R) [Alexa Fluor™ Plus 555] Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide corresponding to TOP2A residues within aa1431-1531 of TOP2A (Uniprot: P11388 )
• Localization: Nuclear
• Marker: Proliferation & Drug-Resistance Marker
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TOP2A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TOP2A Antibody (TOP2A/4397R) [Alexa Fluor™ Plus 555]

• DNA topoisomerase (ATP-hydrolyzing)
• DNA topoisomerase 2-alpha
• DNA topoisomerase II, 170 kD
• DNA topoisomerase II, alpha isozyme
• EC 5.99.1.3
• TOP2
• TOP2A
• TOP2DNA gyrase
• topoisomerase (DNA) II alpha (170kD)
• topoisomerase (DNA) II alpha 170kDa
• TP2A

Background

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOP2A