Recombinant Human TNF RII/TNFRSF1B Protein

Images

 
SDS-Page: Human TNF RII/TNFRSF1B Protein [NBP2-35275]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Func, PAGE
Format
Carrier-Free
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human TNF RII/TNFRSF1B Protein Summary

Description
A single, non-glycosylated biologically active polypeptide chain corresponding to 184 residues of TNFRSF1B.

Source: E. coli

Amino Acid Sequence: MPAQVAFTPY APEPGSTCRL REYYDQTAQM CCSKCSPGQH AKVFCTKTSD TVCDSCEDST YTQLWNWVPE CLSCGSRCSS DQVETQACTR EQNRICTCRP GWYCALSKQE GCRLCAPLRK CRPGFGVARP GTETSDVVCK PCAPGTFSNT TSSTDICRPH QICNVVAIPG NASMDAVCTS TSPT

Preparation
Method
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
Details of Functionality
TNF Receptor II Protein is fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-alpha mediated cytotoxicity in the L-929 cells is less than 0.2 ug/ml, corresponding to a specific activity of > 5000 IU/mg in the presence of 0.25 ng/mL of rHuTNF-alpha.
Protein/Peptide Type
Biologically Active Protein
Gene
TNFRSF1B
Purity
> 97 % pure by SDS-PAGE and HPLC
Endotoxin Note
Less than 0.1 EU/ug of endotoxin as determined by LAL method

Applications/Dilutions

Theoretical MW
20.0 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
Lyophilized from a 0.2 um filtered solution in PBS, pH 7.4.
Concentration
LYOPH
Purity
> 97 % pure by SDS-PAGE and HPLC
Reconstitution Instructions
Reconstitute with sterilized distilled water or 0.1% BSA aqueous buffer to a final concentration of 0.1 - 1.0 mg/ml.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Recombinant Human TNF RII/TNFRSF1B Protein

  • CD120b antigen
  • CD120b
  • Etanercept
  • p75 TNF receptor
  • p75TBPII
  • p75TNFR
  • soluble TNFR1B variant 1
  • sTNF RI
  • sTNF RI/TNFRSF1A
  • TNF RII
  • TNFBRp80 TNF-alpha receptor
  • TNF-R2
  • TNFR2TNFR1B
  • TNF-R75
  • TNFR80
  • TNFRII
  • TNF-RII
  • TNF-R-II
  • TNFR-II
  • TNFRSF1B
  • tumor necrosis factor beta receptor
  • tumor necrosis factor binding protein 2
  • Tumor necrosis factor receptor 2
  • tumor necrosis factor receptor superfamily member 1B
  • tumor necrosis factor receptor superfamily, member 1B
  • Tumor necrosis factor receptor type II

Background

Tumor Necrosis Factor Receptor-I (TNF RI, also known as p55/p60) and TNF RII (also known as p75/p80) are both high affinity receptors for TNF-alpha and -beta, potent mediators of multiple aspects of inflammatory immune responses. Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Whereas all cell types are thought to express TNF RI, TNF RII expression is limited primarily to hematopoietic cells and cells of the immune system. Most of the biological functions of TNF are mediated via TNF RI. Soluble forms of both TNF RI and TNF RII, generated as a result of proteolytic cleavage of the extracellular domains, have been detected in vivo in various biological fluids. The soluble TNF receptors can bind TNF with high affinity and functions as TNF antagonists.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB367
Species: Hu, Ca
Applications: WB, IHC, ICC
248-BD/CF
Species: Hu, Mu, Rt, Ca, Eq
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
H00005034-B01P
Species: Hu
Applications: WB, ICC/IF
MAB7745
Species: Hu
Applications: WB, Simple Western, IHC
MAB6638
Species: Hu, Mu, Rt
Applications: WB
NBP2-60655
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-83227
Species: Hu, Mu(-)
Applications: WB, IHC, IHC-P
H00004354-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IP
NBP1-87801
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF175
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, Neut
7268-CT
NB100-524
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP1-84550
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
7815-NG/CF
Species: Rt
NBP2-35275
Species: Hu
Applications: Func, PAGE

Publications for TNF RII/TNFRSF1B Protein (NBP2-35275) (0)

There are no publications for TNF RII/TNFRSF1B Protein (NBP2-35275).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TNF RII/TNFRSF1B Protein (NBP2-35275) (0)

There are no reviews for TNF RII/TNFRSF1B Protein (NBP2-35275). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

FAQs for TNF RII/TNFRSF1B Protein (NBP2-35275) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TNF RII/TNFRSF1B Products

Bioinformatics Tool for TNF RII/TNFRSF1B Protein (NBP2-35275)

Discover related pathways, diseases and genes to TNF RII/TNFRSF1B Protein (NBP2-35275). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TNF RII/TNFRSF1B Protein (NBP2-35275)

Discover more about diseases related to TNF RII/TNFRSF1B Protein (NBP2-35275).
 

Pathways for TNF RII/TNFRSF1B Protein (NBP2-35275)

View related products by pathway.

PTMs for TNF RII/TNFRSF1B Protein (NBP2-35275)

Learn more about PTMs related to TNF RII/TNFRSF1B Protein (NBP2-35275).

Blogs on TNF RII/TNFRSF1B

There are no specific blogs for TNF RII/TNFRSF1B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-2 [Unconjugated]
202-IL-010/CF
IL-2 [Unconjugated]
202-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant Human TNF RII/TNFRSF1B Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol TNFRSF1B
Entrez
Uniprot